直链淀粉-鹅去氧胆酸接枝聚合物的合成及其自组装行为

doi:10.11901/1005.3093.2020.019

材料研究学报

2020, Vol. 34

Issue (8): 569-574 DOI: 10.11901/1005.3093.2020.019

研究论文

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直链淀粉-鹅去氧胆酸接枝聚合物的合成及其自组装行为

熊校勤(

), 张洪权, 方婵雨

湖北第二师范学院化学与生命科学学院植物抗癌活性物质提纯与应用湖北省重点实验室武汉 430205

Synthesis and Self-assembly Behavior of Amylose-chenodeoxycholic Acid Conjugates

XIONG Xiaoqin(

), ZHANG Hongquan, FANG Chanyu

Hubei Key Laboratory of Purification and Application of Plant Anticancer Active Ingredients, School of Chemistry and Life Sciences, Hubei University of Education, Wuhan 430205, China

引用本文:

熊校勤, 张洪权, 方婵雨. 直链淀粉-鹅去氧胆酸接枝聚合物的合成及其自组装行为[J]. 材料研究学报, 2020, 34(8): 569-574.
Xiaoqin XIONG, Hongquan ZHANG, Chanyu FANG. Synthesis and Self-assembly Behavior of Amylose-chenodeoxycholic Acid Conjugates[J]. Chinese Journal of Materials Research, 2020, 34(8): 569-574.

全文: PDF(3798 KB) HTML

摘要:

使用1-乙基-3-(3－二甲基氨丙基)-碳化二亚胺(EDC)/N-羟基琥珀酰亚胺(NHS)交联剂合成一种直链淀粉接枝鹅去氧胆酸聚合物(Amylose–chenodeoxycholic acid conjugates, AMY-CDCA)，并用傅里叶变换红外光谱(FTIR)、核磁共振氢谱(¹H NMR)和紫外光谱法对其进行了表征。结果表明，CDCA已经成功地接枝到直链淀粉骨架上，接枝度为138.15/100个葡萄糖单元。可用透析法将AMY-CDCA聚合物制备成球形并具有核壳结构的胶束，其平均粒径为224 nm，多分散指数为0.110。使用疏水性荧光探针芘和尼罗红研究了胶束的组装行为。结果表明，聚合物的临界胶束浓度(CMC)为2.8×10^-3 mg/mL，其疏水核心对尼罗红有增溶作用。

关键词 ：有机高分子材料, 直链淀粉, 鹅去氧胆酸, 胶束, 自组装

Abstract：

Amylose-chenodeoxycholic acid conjugates (AMY-CDCA) were prepared using 1-ethyl-3-(3-dimethylaminopropyl)-carbonized diimine (EDC) /N-hydroxysuccinimide (NHS) cross-linking agents. The AMY-CDCA polymer was characterized by FTIR, ¹H NMR and ultraviolet spectroscopy. It was shown that CDCA was successfully coupled to the amylose backbone with a molar substitution of 138.15 per 100 glucose units. Self-Assembled micelles were prepared from the AMY-CDCA polymer through dialysis method. The average particle size of the micelles measured by dynamic light scattering method is 224 nm, and the polydispersity index is 0.110. TEM images demonstrated that the micelles are of spherical shape with a core-shell structure. The critical micelle concentration is 2.8×10^-3 mg/mL, which was determined using a probe fluorescence technique in the presence of pyrene. Additionally, Nile Red can be encapsulated and stabilized in the hydrophobic core of the micelles.

Key words： organic polymer materials amylose chenodeoxycholic acid micelle self assembly

收稿日期: 2020-01-15

ZTFLH:

O631

基金资助:湖北省自然科学基金(2016CFB310)

作者简介: 熊校勤，男，1984年生，博士

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https://www.cjmr.org/CN/10.11901/1005.3093.2020.019 或 https://www.cjmr.org/CN/Y2020/V34/I8/569

图1 AMY-CDCA的合成路线

图2 Amylose、CDCA和AMY-CDCA的红外光谱图

图3 Amylose和AMY-CDCA的1H NMR图谱

图4 CDCA的紫外吸收标准曲线

图5 AMY-CDCA聚合物胶束的的粒径分布

图6 AMY-CDCA聚合物胶束的透射电子显微镜照片

图7 不同浓度的AMY-CDCA胶束溶液对芘的激发光谱中I333/I338 的影响

图8 不同浓度的AMY-CDCA胶束溶液中尼罗红的荧光发射光谱

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