超快温度响应性纳米纤维水凝胶的制备及其用于药物的可控释放

doi:10.11901/1005.3093.2019.493

材料研究学报

2020, Vol. 34

Issue (6): 452-458 DOI: 10.11901/1005.3093.2019.493

研究论文

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超快温度响应性纳米纤维水凝胶的制备及其用于药物的可控释放

郑勰¹^,², 查刘生¹^,²(

)

1.东华大学纤维材料改性国家重点实验室上海 201620
2.东华大学材料科学与工程学院上海 201620

Preparation of Ultra-fast Temperature Responsive Nanofibrous Hydrogel and Application in Controllable Drug Release

ZHENG Xie¹^,², ZHA Liusheng¹^,²(

)

1.State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, China
2.College of Materials Science and Engineering, Donghua University, Shanghai 201620, China

引用本文:

郑勰, 查刘生. 超快温度响应性纳米纤维水凝胶的制备及其用于药物的可控释放[J]. 材料研究学报, 2020, 34(6): 452-458.
Xie ZHENG, Liusheng ZHA. Preparation of Ultra-fast Temperature Responsive Nanofibrous Hydrogel and Application in Controllable Drug Release[J]. Chinese Journal of Materials Research, 2020, 34(6): 452-458.

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摘要:

先使用N-异丙基丙烯酰胺和N-羟甲基丙烯酰胺合成共聚物，以其为成纤聚合物用静电纺丝工艺制备纳米纤维，然后进行高速搅拌将纳米纤维切短并分散在叔丁醇中，最后进行冷冻干燥和热处理将切短的纳米纤维组装成具有多级多孔结构的纳米纤维水凝胶。这种纳米纤维水凝胶在水中具有良好的稳定性、压缩回弹性和显著的温度响应性。当水介质温度在20~55℃交替变化时它具有超快的温度响应性，达到消溶胀平衡的时间为34 s，达到溶胀平衡的时间为45 s。体外药物释放实验结果表明，当pH值为7.4的磷酸盐缓冲溶液的温度在15~47℃交替变化时，其中浸入的载模型药物葡聚糖的温度响应性纳米纤维水凝胶以“开/关”模式可控释放药物。

关键词 ：有机高分子材料, 温度响应性纳米纤维水凝胶, 静电纺丝, 药物可控释放

Abstract：

The nanofibers were firstly prepared by static electrospinning process using the fiber-forming copolymer synthesized from N-isopropylacrylamide and N-methylol acrylamide, and then they were shortened and dispersed in tert-butanol by high-speed stirring. Finally, the shortened nanofibers were assembled into nanofibrous hydrogel with hierarchical porous structure by the processes of freeze drying and followed heat treatment. The resultant nanofibrous hydrogel in aqueous medium holds excellent stability, compression resilience and remarkable temperature-responsiveness. When the temperature of an aqueous medium changed alternately between 20℃ and 55℃, in which the nanofibrous hydrogel reached its swelling- and deswelling-equilibrium state within 34 s and 45 s respectively, exhibiting ultra-fast temperature responsiveness. In vitro drug release experiment results show that when the temperature of the phosphate buffered solution of pH7.4 is altered alternately between 15^oC and 47^oC the immersed dextran (model drug) loaded nanofibrous hydrogel can controllably release the drug by ''on/off'' mode.

Key words： organic polymer materials temperature responsive nanofibrous hydrogel static electrospinning controllable drug release

收稿日期: 2019-10-25

ZTFLH:

TQ430.50

基金资助:国家自然科学基金(51373030)

作者简介: 郑勰，男，1991年生，博士

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https://www.cjmr.org/CN/10.11901/1005.3093.2019.493 或 https://www.cjmr.org/CN/Y2020/V34/I6/452

图1 PNN的1H NMR谱图和质量分数约为0.1%的PNN水溶液的500 nm波长光透过率与温度的关系

图2 PNN纳米纤维不同放大倍数的SEM照片

图3 不同形状温度响应性纳米纤维水凝胶的照片、温度响应性纳米纤维水凝胶不同放大倍数的SEM照片、温度响应性纳米纤维水凝胶在300 r/min转速下振荡24 h前后外观照片、传统的温度响应性PNIPAM水凝胶受到刀片压缩前后及其移除后的外观照片以及温度响应性纳米纤维水凝胶受到刀片压缩前后及其移除后的外观照片

图4 纳米纤维水凝胶和PNIPAM水凝胶的V/V0随温度变化的曲线、纳米纤维水凝胶交替放入20℃和55℃水中测得的V/V0随时间变化的曲线和纳米纤维水凝胶交替6次浸入20℃和55℃水中达到溶胀或收缩平衡后测得的V/V0

图5 负载FITC-葡聚糖的温度响应性纳米纤维水凝胶和传统的PNIPAM水凝胶响应温度在15~47℃间交替变化释放的药物累积量曲线

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