PVP/PCL纳米纤维复合支架的生物活性*

doi:10.11901/1005.3093.2014.213

材料研究学报

2014, Vol. 28

Issue (10): 769-774 DOI: 10.11901/1005.3093.2014.213

本期目录 | 过刊浏览

PVP/PCL纳米纤维复合支架的生物活性*

刘淑琼¹,肖秀峰²(

)

1. 武夷学院生态与资源工程学院武夷山 354300
2. 福建师范大学化学与化工学院福州 350007

Cytocompatibility of PCL/PVP Composite Nanofibrous Scaffolds

Shuqiong LIU¹,Xiufeng XIAO^2,^**(

)

1. Ecology and Resource Engineering, Wuyi University, Wuyishan 354300
2. College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350007

引用本文:

刘淑琼,肖秀峰. PVP/PCL纳米纤维复合支架的生物活性*[J]. 材料研究学报, 2014, 28(10): 769-774.
Shuqiong LIU, Xiufeng XIAO. Cytocompatibility of PCL/PVP Composite Nanofibrous Scaffolds[J]. Chinese Journal of Materials Research, 2014, 28(10): 769-774.

全文: PDF(3934 KB) HTML

摘要:

以二氧六环/水为溶剂体系, 以聚己内酯(PCL)和聚乙烯吡咯烷酮(PVP)为原料, 采用相分离法制备纳米纤维支架, 研究了陈化、溶剂比例和PVP含量对支架的纳米纤维结构形成的影响, 以及PVP的添加对支架生物活性和亲水性能的影响。结果表明, 陈化直接影响纳米纤维网络结构的形成; 溶剂体系和PVP含量均影响纳米纤维结构, 随着PVP含量的提高纳米纤维结构逐渐消失。随着PVP含量的提高, 支架的亲水性能提高。PVP/PCL复合纳米纤维支架浸泡模拟体液(SBF)后在其内部和表面皆生成碳磷灰石(CHA)涂层, 表明该纳米纤维支架具有良好的生物活性, 且PVP/PCL支架比纯PCL支架能更快诱导生成碳磷灰石。

关键词 ：有机高分子材料, 聚己内酯, 聚乙烯吡咯烷酮, 陈化, 纳米纤维, 相分离

Abstract：

By using polycarpolactone (PCL) and polyvinylpyrrolidone (PVP) as raw materials, a series of nanofibrous scaffolds were fabricated by thermally induced phase separation in a dioxane/water system. The effect of aging temperature, PVP content and different ratios of dioxane to water on the morphology of nanofibrous scaffolds was investigated. The effect of PVP content on the biological activity and hydrophilic performance of the prepared nanofibrous scaffolds were also examined. The results show that aging process played a crucial role in forming the unique nanofibrous structure. The solvent system and the PVP content affect the nanofibrous structure, peculiarly; the nanofibrous structure would disappear gradually with the increasing PVP content. The result of the water absorption shows that the hydrophilic performance of the nanofibrous stent increases with the increasing PVP content. Furthermore, the test of biological activity shows that there exists crystallite carbonate hydroxyapatite in the scaffolds, indicating that the PCL/PVP nanofiberous scaffolds have a good biological activity, and which may much rapidly facilitate the forming of crystallite carbonate hydroxyapatite rather than the merely PCL ones.

Key words： organic polymer materials polycarpolactone polyvinylpyrrolidone aging nanofibrous phase separation

收稿日期: 2014-04-25

基金资助:* 国家自然科学基金30970887和武夷学院青年教师专项科研基金xq201102资助项目。

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链接本文:

https://www.cjmr.org/CN/10.11901/1005.3093.2014.213 或 https://www.cjmr.org/CN/Y2014/V28/I10/769

图1 有无陈化的PVP/PCL复合支架电镜照片(a) 直接-20℃冷冻2 h, (b) 先0℃凝胶3 h, 再-20℃冷冻2 h

图2 不同溶剂体系的PVP/PCL复合支架电镜照片

图3 不同PVP含量的PCL/PVP复合支架的电镜照片

图4 不同PVP含量的PCL/PVP复合支架浸泡模拟体液14 d后的电镜照片

图5 20%PVP含量的PCL/PVP复合支架浸泡模拟体液不同时间后的电镜照片

图6 PCL/PVP(30%) 支架浸泡模拟体液不同时间后的FTIR谱图

表1 不同条件的支架孔隙率、吸水率

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