新型磷镁晶须的体外生物相容性和抗菌性能*

doi:10.11901/1005.3093.2015.643

材料研究学报

2016, Vol. 30

Issue (3): 220-228 DOI: 10.11901/1005.3093.2015.643

本期目录 | 过刊浏览

新型磷镁晶须的体外生物相容性和抗菌性能*

赵冰^1,^2,^4,⁵, 徐大可³, 孙子晴³, 任伊宾³, 战德松^1,^2,^4,⁵(

), 肖克沈³, 杨柯³

1. 中国医科大学附属口腔医学院材料教研室沈阳 110002
2. 辽宁省口腔医院研究所沈阳 110002
3. 中国科学院金属研究所沈阳 110016
4. 辽宁省口腔疾病重点实验室沈阳 110002
5. 辽宁省口腔疾病转化医学研究中心沈阳 110002

In Vitro Biocompatibility and Antibacterial Property of a Novel Magnesium Phosphate Whisker

ZHAO Bing^1,^2,^4,⁵, XU Dake³, SUN Ziqing³, REN Yibin³, ZHAN Desong^1,^2,^4,^5,^**(

), XIAO Keshen^3,^**, YANG Ke³

1. Department of Dental Material, School of Stomatology, China Medical University, Shenyang 110002, China
2. Liaoning Institute of Dental Research, Shenyang 110002, China
3. Institute of Metal Research, Chinese Academic of Sciences, Shenyang 110016, China
4. Liaoning Province Oral Diseases Key Laboratory, Shenyang 110002, China
5. Liaoning Province Oral Diseases Translation Medcicne Research Center, Shenyang 110002, China;

引用本文:

赵冰, 徐大可, 孙子晴, 任伊宾, 战德松, 肖克沈, 杨柯. 新型磷镁晶须的体外生物相容性和抗菌性能*[J]. 材料研究学报, 2016, 30(3): 220-228.
Bing ZHAO, Dake XU, Ziqing SUN, Yibin REN, Desong ZHAN, Keshen XIAO, Ke YANG. In Vitro Biocompatibility and Antibacterial Property of a Novel Magnesium Phosphate Whisker[J]. Chinese Journal of Materials Research, 2016, 30(3): 220-228.

全文: PDF(3381 KB) HTML

摘要:

采用RTCA法和Annexin-V/PI双标记法研究了一种新型磷镁晶须的体外生物相容性, 采用共培养法研究了抗菌性能.结果表明, 磷镁晶须材料的生物相容性随着晶须浓度的提高而降低, 晶须浓度小于等于500 μg/mL 时具有较好的体外生物相容性.当晶须浓度为50 μg/mL 和 200 μg/mL时, 对成骨细胞几乎没有影响.磷镁晶须的杀菌效能随着浓度的提高而提高, 浓度为500 μg/mL时对大肠杆菌和金黄色葡萄球菌的杀菌率分别达到96.84%和99.93%, 表现出优异的抗菌性能.

关键词 ：无机非金属材料, 磷镁晶须, 生物相容性, 抗菌性能, 细胞凋亡, RTCA

Abstract：

The in vitro biocompatibility of a novel magnesium phosphate whisker (M-PW) was evaluated by real-time cellular analysis (RTCA) and Annexin-V/PI double marking methods, and its antibacterial property was evaluated by co-culture method. Results show that the in vitro biocompatibility of the M-PW decreased with the increase of M-PW in the suspensions. It possessed excellent in vitro biocompatibility for the suspensions containing 500 μg/mL or lower amount of the M-PW. It had no toxic effect on osteoblast cells for the suspension with 50 and 200 μg/mL of M-PW respectively. The antibacterial efficacy of the suspensions increased with the increasing amount of M-PW. The antibacterial efficacy against Escherichia coli and Staphylococcus aureus achieved 96.84% and 99.93% respectively for the suspension with 500 μg/mL of M-PW, demonstrating that the novel phosphorous-magnesium whisker possesses excellent antibacterial property.

Key words： inorganic non-metallic materials magnesium phosphate whisker biocompatibility antibacterial property apoptosis RTCA

收稿日期: 2015-11-17

ZTFLH:

TB321

基金资助:* 沈阳市科技计划F16-206-9-14资助项目

作者简介: 战德松, 教授

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图1 磷镁晶须的扫描电镜照片

表1 加入MC3T3-E1细胞前各α-MEM晶须液组和空白对照组的pH值 (n=5)

图2 4组不同浓度的磷镁晶须和空白组对MC3T3-E1细胞生长影响的实时细胞分析

表2 MC3T3-E1细胞在4组浓度的磷镁晶须作用下的细胞相对增值率

表3 4组不同浓度的磷镁晶须对MC3T3-E1细胞毒性等级

图3 用Annexin V/PI 流式细胞仪分析检测的48 h MC3T3-E1细胞凋亡

表4 各组细胞培养48 h后流式细胞仪检测的凋亡率

表5 4组浓度的磷镁晶须对大肠杆菌和金黄色葡萄球菌的杀菌率(n=5)

图4 各组磷镁晶须对大肠杆菌和金黄色葡萄球菌作用效果的照片

图5 磷镁晶须杀菌过程的示意图

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