胶原组装形态对仿生矿化的影响*

doi:10.11901/1005.3093.2015.286

材料研究学报

2016, Vol. 30

Issue (1): 51-56 DOI: 10.11901/1005.3093.2015.286

研究论文

本期目录 | 过刊浏览

胶原组装形态对仿生矿化的影响*

丁珊^1,²(

), 唐敏健³, 陈俊杰¹, 周长忍^1,², 李立华^1,², 李红^1,²

1. 暨南大学材料科学与工程系广州 510632
2. 人工器官与材料教育部工程研究中心广州 510632
3. 国家知识产权局专利局专利审查协作广东中心广州 510530

Effects of Collagen Assembly Form on Biomimetic Mineralization

DING Shan^1,^2,^**(

), TANG Minjian³, CHEN Junjie¹, ZHOU Changren^1,², LI Lihua^1,², LI Hong^1,²

1. Department of Material Science and Engineering, Jinan University, Guangzhou 510632, China
2. Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, China
3. Patent Examination Cooperation Center of the Patent Office, SIPO, Guangzhou 510530, China

引用本文:

丁珊, 唐敏健, 陈俊杰, 周长忍, 李立华, 李红. 胶原组装形态对仿生矿化的影响*[J]. 材料研究学报, 2016, 30(1): 51-56.
Shan DING, Minjian TANG, Junjie CHEN, Changren ZHOU, Lihua LI, Hong LI. Effects of Collagen Assembly Form on Biomimetic Mineralization[J]. Chinese Journal of Materials Research, 2016, 30(1): 51-56.

全文: PDF(4628 KB) HTML

摘要:

模拟天然骨组织中胶原的有序结构, 构建多级有序的胶原液晶膜, 以胶原非液晶膜作为参照, 通过体外的仿生矿化考察了胶原液晶模板对于仿生矿化的影响。采用偏光显微镜、扫描电镜、原子力显微镜、X-射线衍射等手段分析了仿生矿化不同时间后在两种模板上沉积的钙磷盐的形貌与结构。结果表明, 胶原液晶的多级有序结构对仿生矿化有比较明显的影响; 与胶原非液晶模板相比, 在胶原液晶模板上沉积的钙磷盐不仅在微观上有序, 而且在宏观上也很有序且致密。这表明, 胶原液晶的这种多级有序结构有利于构建仿生骨组织修复材料。

关键词 ：复合材料, 胶原, 液晶, 仿生矿化, 有序结构

Abstract：

To simulate the ordered texture of the collagen in natural bone tissue, membranes of collagen with hierarchically ordered texture were fabricated. The effect of templates made of collagen with and without ordered texture on the biomineralization process of calcium phosphate was comparatively studied in vitro. The morphology and structure of the deposited calcium phosphate on the collagen templates were characterized by using polarizing microscope (POM), scanning electron microscope (SEM), atomic force microscope (AFM) and X-ray diffraction (XRD). The results show that the collagen with hierarchically ordered texture has obvious effect on the biomimetic mineralization process; on which the deposited calcium phosphate is much more orderly and compact either in micro- or in macro scales. This result indicates that the collagen with hierarchically ordered texture is beneficial to the building of biomimetic bone tissues as materials for repair of human bone etc.

Key words： composite collagen liquid crystal biomineralization ordered texture

收稿日期: 2015-05-13

基金资助:* 国家自然科学基金31400824和31270021资助项目

作者简介: 丁珊

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https://www.cjmr.org/CN/10.11901/1005.3093.2015.286 或 https://www.cjmr.org/CN/Y2016/V30/I1/51

图1 40 mg/mL胶原液晶膜仿生矿化不同时间后的偏光显微镜观察

图2 40 mg/mL的胶原液晶膜仿生矿化6 h经茜素红染色后的偏光显微镜观察

图3 胶原膜仿生矿化不同时间后的AFM观察

图4 两种胶原膜仿生矿化24 h后的SEM观察

图5 两种胶原膜仿生矿化不同时间后的XRD分析

图6 胶原膜仿生矿化不同时间后SBF溶液中的Ca、P含量

表1 胶原膜仿生矿化不同时间后膜表面的Ca与P比

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