钛(IV)配合物催化合成Ti-P聚乳酸材料的亲水性、降解率和细胞毒性*

doi:10.11901/1005.3093.2013.788

材料研究学报

2014, Vol. 28

Issue (5): 395-400 DOI: 10.11901/1005.3093.2013.788

本期目录 | 过刊浏览

钛(IV)配合物催化合成Ti-P聚乳酸材料的亲水性、降解率和细胞毒性*

胡承波(

)

重庆文理学院环境材料与修复技术重庆市重点实验室重庆 402160

Hydrophilicity Degradability and Cell Toxicity of Ti-P Polylactide Paterial Synthesized by Titanium (IV) Complex as Catalyst

Chengbo HU(

)

Chongqing Key Laboratory of Environmental Materials & Remediation Technologies, Chongqing University of Arts and Sciences, Chongqing 402160

引用本文:

胡承波. 钛(IV)配合物催化合成Ti-P聚乳酸材料的亲水性、降解率和细胞毒性*[J]. 材料研究学报, 2014, 28(5): 395-400.
Chengbo HU. Hydrophilicity Degradability and Cell Toxicity of Ti-P Polylactide Paterial Synthesized by Titanium (IV) Complex as Catalyst[J]. Chinese Journal of Materials Research, 2014, 28(5): 395-400.

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摘要:

以双(烷氧-亚胺芳氧)基钛(IV)配合物(BAIP-Ti(IV))为催化剂催化D, L-丙交酯开环聚合合成的Ti-P聚乳酸, 用静态水接触角、吸水率及降解实验考察其理化性能, 以小鼠颅顶前骨细胞(MC3T3-E1)考察其在材料表面的增值和黏附铺展情况。结果表明: Ti-P聚乳酸比Sn-P聚乳酸有较低的亲水性和较高的抗水解性; MC3T3-E1细胞在Ti-P聚乳酸材料表面显示出的较好的增值活力以及细胞黏附与铺展能力; 在Ti-P聚乳酸中残存的微量Ti(IV)金属配合物对MC3T3-E1细胞无细胞毒性, 不影响MC3T3-E1细胞在其材料表面上生长。

关键词 ：有机高分子材料, 配合物, 丙交酯, 聚乳酸, 降解, 生物相容性

Abstract：

Ti-P polylactide was synthesized by ring-opening polymerization (ROP) of D, L-lactide with bis-(alkoxy-imine-phenoxy) titanium (IV) complex as catalyst. The physico-chemical properties of the Ti-P material were investigated by measurements of contact angle, water absorption rate and degradability, while its biocompatibility to MC3T3-E1 cells, such as the proliferation, adhesion and spreading performance of murine preosteoblastic cells (MC3T3-E1) was also investigated. The results show that the hydrophilicity of Ti-P polylactide is weaker than Sn-P polylactide, inversely, its anti-hydrolysis ability is stronger. In contact with the Ti-P polylactide material, the MC3T3-E1 cells showed excellent activity in proliferation, adhesion and spreading. The rudimental Ti(IV) complex in the Ti-P polylactide exihibits non-toxicity to MC3T3-E1 cell and does not hamper the growth of MC3T3-E1 cells on the surface of the Ti-P polylactide material.

Key words： organic polymer materials complex lactide polylactide degradation biocompatibility

收稿日期: 2013-08-25

基金资助:* 重庆市自然科学基金cstc2013jcyjA50022 和重庆文理学院重点项目Z2013CH07。

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https://www.cjmr.org/CN/10.11901/1005.3093.2013.788 或 https://www.cjmr.org/CN/Y2014/V28/I5/395

表1 样品的静态水接触角和24 h的吸水率

图1 两种材料表面静态水接触角 (A: Sn-P, B: Ti-P)

图2 材料降解失重率随时间的变化(n=6)

图3 在材料降解过程中pH值的变化(n=6)

图4 MTT检测MC3T3-E1细胞在不同材料上的增殖情况(490 nm)

图5 培养不同时间的MC3T3-E1细胞在Glass(A)、Sn-P(B)以及Ti-P(C)表面的激光共聚焦成像 (A1, B1, C1)24 h; (A2, B2, C2)48h

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