材料研究学报  2011, Vol. 25 Issue (3): 243-248

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多级开孔壳聚糖海绵的细胞行为分析

赵名艳1,2,  李立华1,2,  周长忍1,2,  李贤1,2

1.暨南大学材料科学与工程系 广州 510632 2.人工器官与材料教育部工程中心 广州 510632

Analysis of Cell Behaviors in Chitosan Sponges with Hierarchical Open Pores

ZHAO Mingyan1,2, LI Lihua1,2, ZHOU Changren1,2, LI Xian1,2

1.Department of Material Science and Engineering, Jinan University, Guangzhou 510632 2.Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632

赵名艳 李立华 周长忍 李贤. 多级开孔壳聚糖海绵的细胞行为分析[J]. 材料研究学报, 2011, 25(3): 243-248.
, , , . Analysis of Cell Behaviors in Chitosan Sponges with Hierarchical Open Pores[J]. Chin J Mater Res, 2011, 25(3): 243-248.

摘要 参考文献 相关文章 Metrics 全文: PDF(936 KB)   摘要: 使用冰滴为致孔剂制备表面大孔、内部孔洞相连的新型壳聚糖(HPCS)支架, 将其与聚乳酸复合制备出三维蜂窝状孔洞结构的复合支架(THCP)。对HPCS和THCP进行了表面形貌、力学性能、细胞相容性等方面的表征, 并与常规冻干法所制备的壳聚糖(CS)海绵进行了对比。结果表明, 在HPCS海绵表面均匀分布着大而开放的孔洞, 大孔内部形成相互贯通的小孔, 在THCP复合海绵内部形成了相互贯通的蜂窝状结构。THCP支架的力学强度比纯壳聚糖支架有明显的提高, 其细胞活性及细胞增殖指数等都明显优于HPCS及CS海绵。因此, 对壳聚糖拓扑结构的这种设计不仅弥补了常规冻干支架的缺陷, 提高了细胞相容性, 还将表面图案化改性应用延伸到了三维尺度。 关键词 ： 有机高分子材料,  壳聚糖,  多级开孔支架,  蜂窝状结构,  细胞相容性     Abstract：A novel hierarchically porous chitosan (HPCS) sponge with large open pores and interconnected small pores on the surface was fabricated with ice particles as poroen. The honeycomb patterned porous composite scaffolds (HPCS) was obtained by immersing the sponges into poly (L-lactic acid) chloroform solution. A comparison of the morphology, mechanical properties and cell compatibility with the three different sponges were made. Results show that honeycomb patterned porous structure is visible in the THCP sponges, wherein interconnected micro pores embedded in larger open pores and formed uniform network. Hierarchically porous structure is visible in the HPCS sponges. The compressive strength of the THCP sponges dramatically increased, cell viability and cell proliferation index (PI) are higher than that of the CS and HPCS sponges. The topographical design of chitosan scaffolds remedied the traditional freeze-drying technique, improved cell biocompatibility and elongated the design of topological structure into the three dimension area. Key words： organic polymer materials    chitosan    hierarchically porous scaffold    honeycomb patterned structure    cell compatability 收稿日期: 2011-03-03      ZTFLH:  R318   基金资助: 国家自然科学基金30900307、教育部博士点基金255820和广东省自然科学基金博士启动项目9451063201002964资助。 服务 把本文推荐给朋友 加入引用管理器 E-mail Alert RSS 作者相关文章 赵名艳 李立华 周长忍 李贤 44.8K 链接本文: https://www.cjmr.org/CN/      或      https://www.cjmr.org/CN/Y2011/V25/I3/243 1 S.Pakakrong, S.Orawan, N.Artphop, C.Poonlarp, P.Prasit, S.Pitt, In vitro biocompatibility of electrospun and solvent-cast chitosan substrata towards Schwann, osteoblast, keratinocyte and fibroblast cells, European, Polymer Journal, 3(46), 428(2010) 2 W.Teo, W.He, S.Ramakrishna, Electrospun scaffold tailored for tissue-specific extracellular matrix, Biotechnol., 1(9), 918(2006) 3 I.Kim, S.Seo, H.Moon, M.Yoo, I.Park, B.Kim, C.Cho, Chitosan and its derivatives for tissue engineering applications, Biotechnol. 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