<strong>Ti-Zr-Cu</strong>合金的抗菌性能和体外生物相容性

doi:10.11901/1005.3093.2020.582

材料研究学报

2021, Vol. 35

Issue (11): 873-880 DOI: 10.11901/1005.3093.2020.582

研究论文

本期目录 | 过刊浏览

Ti-Zr-Cu合金的抗菌性能和体外生物相容性

于佳莹¹^,²^,⁴, 杨希祥¹^,²^,⁴, 战德松¹^,²^,⁴(

), 杨柯³, 任玲³, 王敬人¹^,²^,⁴, 徐嘉蔚¹^,²^,⁴

^1.中国医科大学附属口腔医学院材料教研室沈阳 110002
^2.辽宁省口腔医院研究所沈阳 110002
^3.中国科学院金属研究所沈阳 110819
^4.辽宁省口腔疾病重点实验室沈阳 110002

Antibacterial Property and in vitro Biocompatibility of a Ti-Zr-Cu Alloy

YU Jiaying¹^,²^,⁴, YANG Xixiang¹^,²^,⁴, ZHAN Desong¹^,²^,⁴(

), YANG Ke³, REN Ling³, WANG Jingren¹^,²^,⁴, XU Jiawei¹^,²^,⁴

^1.Department of Dental Material, School of Stomatology, China Medical University, Shenyang 110002, China
^2.Liaoning Institute of Dental Research, Shenyang 110002, China
^3.Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China
^4.Liaoning Province Oral Diseases Key Laboratory, Shenyang 110002, China

引用本文:

于佳莹, 杨希祥, 战德松, 杨柯, 任玲, 王敬人, 徐嘉蔚. Ti-Zr-Cu合金的抗菌性能和体外生物相容性[J]. 材料研究学报, 2021, 35(11): 873-880.
Jiaying YU, Xixiang YANG, Desong ZHAN, Ke YANG, Ling REN, Jingren WANG, Jiawei XU. Antibacterial Property and in vitro Biocompatibility of a Ti-Zr-Cu Alloy[J]. Chinese Journal of Materials Research, 2021, 35(11): 873-880.

全文: PDF(13136 KB) HTML

摘要:

采用平板共培养法测定Ti-Zr-Cu合金表面的菌落数，通过CCK8细胞增殖检测、鬼笔环肽细胞染色观察细胞核和细胞骨架形貌、细胞凋亡和黏附能力测定，研究了Ti-Zr-Cu合金的抗菌性能和体外生物相容性。结果表明，空白样品和Ti-Zr合金表面上的菌落密集，而Ti-Zr-Cu合金表面上的菌落数极少。Ti-Zr-Cu合金对金黄色葡萄球菌和大肠杆菌的抗菌率分别达到98.28%和97.67%，表现出优异的抗菌性能。MC3T3-E1在Ti-Zr-Cu合金表面培养1、4、7 d的相对增值率分别为168.8%、109.8%和106.5%，均高于100%，表明这种合金没有细胞毒性。细胞在Ti-Zr-Cu合金表面上的附着和铺展良好，有利于贴壁细胞在其表面黏附和进一步增值，表明Ti-Zr-Cu合金具有良好的生物相容性。Ti-Zr-Cu合金对细胞凋亡没有不良影响。从Ti-Zr-Cu合金表面细胞的SEM照片可见细胞的粘附正常，也表明其具有良好的生物相容性。

关键词 ：金属材料, Ti-Zr-Cu合金, 抗菌性能, 生物相容性

Abstract：

The antibacterial property and in vitro biocompatibility of a novel Ti-Zr-Cu alloy were investigated. Meanwhile, the number of bacterial colonies on the surface of Ti-Zr-Cu alloy was determined by plate co-culture method, and the morphology of nuclear and cytoskeleton, cell apoptosis and adhesion ability on Ti-Zr-Cu alloy were assessed by means of CCK8 cell proliferation detection and phyllopeptide cell staining observation. The results show that the density of bacterial colonies on the blank sample and Ti-Zr alloy surface was very high, while the number of bacterial colonies on the Ti-Zr-Cu alloy surface was very low. The antibacterial rate of Ti-Zr-Cu alloy against Staphylococcus aureus and Escherichia coli reached 98.28% and 97.67%, respectively, showing excellent antibacterial properties. The relative productivity of MC3T3-E1 cultured on Ti-Zr-Cu alloy surface for 1, 4 and 7 days were 168.8%, 109.8% and 106.5%, respectively, which were all higher than 100%, indicating that this alloy had no cytotoxicity. The adhesion and spread of cells on the surface of Ti-Zr-Cu alloy are good, which is conducive to the adhesion and further growth of adherent cells on the surface of Ti-Zr-Cu alloy, indicating that Ti-Zr-Cu alloy has good biocompatibility. Ti-Zr-Cu alloy had no adverse effect on cell apoptosis. The SEM images of the cells on the surface of Ti-Zr-Cu alloy showed that the adhesion of the cells was normal, which also indicated that the cells had good biocompatibility.

Key words： metal material Ti-Zr-Cu alloy antibacterial property biocompatibility

收稿日期: 2021-01-05

ZTFLH:

R318

基金资助:国家自然科学基金(51631009);辽宁省科技计划(ZF2019032);沈阳市科技创新研发计划(19-112-4-028)

作者简介: 于佳莹，女，1995年生，硕士生

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https://www.cjmr.org/CN/10.11901/1005.3093.2020.582 或 https://www.cjmr.org/CN/Y2021/V35/I11/873

表1 Ti-Zr合金和Ti-Zr-Cu合金的化学成分

表2 细胞毒性的分级标准和评定结果

图1 金黄色葡萄球菌(S. aureus)和大肠杆菌(E. coli)分别与Ti-Zr合金和Ti-Zr-Cu合金共培养24 h后存活细菌菌落的照片

表3 不同材料对金黄色葡萄球菌和大肠杆菌的杀菌率(n=4)

图2 CCK8细胞增值检测给出的MC3T3-E1细胞的 OD值(在450 nm处的吸光度)

图3 MC3T3-E1细胞在Ti-Zr合金(a)和Ti-Zr-Cu合金(b)表面培养24 h后的罗丹明鬼笔环肽骨架染色

图4 MC3T3-E1 细胞在Ti-Zr合金和Ti-Zr-Cu合金表面培养72 h后的流式散点图和柱状图统计分析

图5 MC3T3-E1细胞在Ti-Zr合金和Ti-Zr-Cu合金表面培养72 h后的扫描电镜照片

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