羟基磷灰石接枝壳聚糖表面改性及其复合水凝胶的生物相容性*

doi:10.11901/1005.3093.2015.154

材料研究学报

2015, Vol. 29

Issue (11): 801-806 DOI: 10.11901/1005.3093.2015.154

本期目录 | 过刊浏览

羟基磷灰石接枝壳聚糖表面改性及其复合水凝胶的生物相容性*

杨慎宇¹,唐三元²,谭文成³,曾戎¹,杨辉²,黄馨霈³,夏吉生⁴,屠美¹(

)

1. 暨南大学材料科学与工程系广州 510632
2. 珠海市人民医院珠海 519000
3. 澳门仁和医疗中心澳门
4. 澳门仁伯爵综合医院澳门

Surface Modification of Hydroxyapatite-Grafted-Chitosan and Biocompatibility Evaluation of CS/HA-G-CS Composite Hydrogel

Shenyu YANG¹,Sanyuan TANG²,Manseng TAM³,Rong ZENG¹,Hui YANG²,Hsiungpei HUANG³,Katsang HA⁴,Mei TU^1,^**(

)

1. Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China
2. Zhuhai People’s Hospital, Guangzhou, Zhuhai 519000, China
3. Centro Medico Ian Wo, Macau, China
4. Centro Hospitalar Conde de São Januário, Macau, China

引用本文:

杨慎宇,唐三元,谭文成,曾戎,杨辉,黄馨霈,夏吉生,屠美. 羟基磷灰石接枝壳聚糖表面改性及其复合水凝胶的生物相容性*[J]. 材料研究学报, 2015, 29(11): 801-806.
Shenyu YANG, Sanyuan TANG, Manseng TAM, Rong ZENG, Hui YANG, Hsiungpei HUANG, Katsang HA, Mei TU. Surface Modification of Hydroxyapatite-Grafted-Chitosan and Biocompatibility Evaluation of CS/HA-G-CS Composite Hydrogel[J]. Chinese Journal of Materials Research, 2015, 29(11): 801-806.

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摘要:

用壳聚糖修饰HA表面制备HA-接枝-壳聚糖纳米羟基磷灰石(HA-g-CS), 然后将其与壳聚糖共混制成CS/HA-g-CS复合水凝胶。FTIR、TGA、XRD的测试结果表明, CS已经成功地接枝到HA的表面, 接枝率为15.8%; SEM结果表明, HA-g-CS在CS 基体的分散性相对于HA得到明显的改善, 且CS/HA-g-CS比CS/HA复合水凝胶的抗压强度提高了43%。CS/HA-g-CS生物相容性评价的结果表明, 材料的细胞毒性和植入安全性均达到了国家标准要求。这表明, CS/HA-g-CS复合水凝胶可作为一种优良的支架材料应用于组织工程领域。

关键词 ：有机高分子材料, 壳聚糖, 羟基磷灰石, 表面改性, 复合水凝胶, 生物相容性

Abstract：

For further improvement of the compatibility between chitosan(CS) and hydroxyapatite (HA), surface modification of HA was carried out with CS to prepare hydroxyapatite-grafted-chitosan (HA-g-CS) The results of FT-IR, TGA and XRD show that CS was successfully grafted onto HA surface, SEM observation showed that the dispersity of HA-g-CS in the CS matrix was significantly improved, and the CS/HA-g-CS hydrogel exhibited much more better compression performance than that of the CS/HA. The biocompatibility evaluation showed that the CS/HA-g-CS composite hydrogel showed no cytotoxicity and implantation safety in vitro according with the requirement of national standards, and was expected to be served as a potential scaffold material applied in tissue engineering.

Key words： organic polymer materials chitosan hydroxyapatite surface modification composite hydrogel biocapatibility

收稿日期: 2015-03-25

基金资助:* 澳门科学技术发展基金072/2013/A, 广东省产业技术研究与开发专项资金项目计划2013B021800115, 珠海市科工贸信局项目2013032218, 广东省高等学校科技创新重点项目CXZD1015和广州市科技计划项目201508020035资助。

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https://www.cjmr.org/CN/10.11901/1005.3093.2015.154 或 https://www.cjmr.org/CN/Y2015/V29/I11/801

图1 羟基磷灰石接枝壳聚糖的反应机理示意图

图2 HA和HA-g-CS粉末XRD谱图

图3 HA、HA-APTES、HA-APTES-GD和HA-g-CS的FT-IR光谱图

图4 HA和HA-g-CS的热重分析

图5 CS/HA和CS/HA-g-CS干凝胶SEM图

表1 凝胶的抗压强度

表2 CS/HA-g-CS材料MTT法细胞毒性结果

图6 CS/HA-g-CS、阴性对照以及阳性对照细胞形态观察

图7 不同植入时间HE染色切片照片

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