嗜硫性顺磁微球的制备及对人血清抗体的分离

材料研究学报

2009, Vol. 23

Issue (1): 93-97

研究论文

本期目录 | 过刊浏览

嗜硫性顺磁微球的制备及对人血清抗体的分离

钱浩; 李春蛟; 张莹雪; 林志勇

华侨大学材料科学与工程学院泉州 362021

Preparation of the thiophiolic paramagnetic polymer microsphere and its isolation of the antibody from human serum

QIAN Hao; LI Chunjiao; ZHANG Yingxue; LIN Zhiyong

College of Material Science and Engineering; Huaqiao University; Quanzhou 362021

引用本文:

钱浩李春蛟张莹雪林志勇. 嗜硫性顺磁微球的制备及对人血清抗体的分离[J]. 材料研究学报, 2009, 23(1): 93-97.
, . Preparation of the thiophiolic paramagnetic polymer microsphere and its isolation of the antibody from human serum[J]. Chin J Mater Res, 2009, 23(1): 93-97.

全文: PDF(843 KB)

摘要:

用微悬浮共聚合技术实现了二乙烯基苯与醋酸乙烯酯单体对亲油性Fe₃O₄纳米粒子的充分包裹, 制备出超顺磁性的聚合物微球, 研究了分离人血清抗体的特异性.结果表明, 聚合物微球的粒径为0.6--20μm, 磁性微球的含量高于24%,饱和磁化强度为16.7 emu/g. 通过充分的水解反应, 在微球表面形成丰富的羟基,并通过Michael加成反应, 在磁性微球表面偶联嗜硫基团(乙烯基砜和巯基乙醇).用嗜硫性磁性微球直接从人血清中分离免疫球蛋白G, 表现出明显的特异性吸附行为.在分离过程中, 吸附行为表现出明显的盐依赖性, 硫酸钠能有效促进其特异性吸附能力.这种吸附行为对温度比较敏感, 在低温条件下吸附能力和分离效率皆降低.利用磁性微球技术的抗体纯化过程, 工艺条件简单, 分离条件温和, 抗体纯度较高,在规模化分离抗体方面表现出较大的潜力.

关键词 ：有机高分子材料, 磁性微球, 微悬浮聚合, 免疫球蛋白G, 分离

Abstract：

The paramagnetic polymer particles with the size range of 0.6–20 μm were synthesized by microsuspension copolymerization with vinyl acetate(VAc) and divinylbenzene(DVB) in the presence of modified magnetite nanoparticles. Evident paramagnetic behavior was observed and saturation magnetization reached 16.7 emu·g−1 at room temperature. These magnetic microspheres were first activated by divinylsulfone, and then modified with mercaptoethanol. By using these thiophilic magnetic microspheres, immunoglobulin G (IgG) could be directly isolated from human serum. This thiophilic magnetic adsorbent performed an evident salt-dependent adsorption behavior for IgG. Due to their strong specificity towards IgG under high salt concentration, the absorbed antibodies could be extracted with high purity. The main factors to influence the isolation, such as the ion types, the ion concentration and the temperature, were also investigated. The technology has great potential to purify antibodies from human serum.

Key words： organic polymer materials magnetic beads immunoglobulin G microsuspension polymerization isolation

收稿日期: 2008-06-25

ZTFLH:

TB324

基金资助:

国家自然科学基金50603007, 50673030和福建省高等学校新世纪优秀人才支持计划资助项目07FJRC02.

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https://www.cjmr.org/CN/ 或 https://www.cjmr.org/CN/Y2009/V23/I1/93

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