3D打印医用钛合金的抗菌性能和体外生物相容性

doi:10.11901/1005.3093.2018.548

材料研究学报

2019, Vol. 33

Issue (2): 117-123 DOI: 10.11901/1005.3093.2018.548

本期目录 | 过刊浏览

3D打印医用钛合金的抗菌性能和体外生物相容性

李改明^1,^2,^4,⁵,刘思雨^1,^2,^4,⁵,战德松^1,^2,^4,⁵(

),刘蕊³,任玲³,杨柯³,王敬人^1,^2,^4,⁵,王强^1,^2,^4,⁵

1. 中国医科大学附属口腔医学院材料教研室沈阳 110002
2. 辽宁省口腔医院研究所沈阳 110002
3. 中国科学院金属研究所沈阳 110819
4. 辽宁省口腔疾病重点实验室沈阳 110002
5. 辽宁省口腔疾病转化医学研究中心沈阳 110002

Antibacterial Properties and Biocompatibility of SLM-fabricated Medical Titanium Alloys

Gaiming LI^1,^2,^4,⁵,Siyu LIU^1,^2,^4,⁵,Desong ZHAN^1,^2,^4,⁵(

),Rui LIU³,Ling REN³,Ke YANG³,Jingren WANG^1,^2,^4,⁵,Qiang WANG^1,^2,^4,⁵

1. Department of Dental Material, School of Stomatology, China Medical University, Shenyang 110002, China
2. Liaoning Institute of Dental Research, Shenyang 110002, China
3. Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China
4. Liaoning Province Oral Diseases Key Laboratory, Shenyang 110002, China
5. Liaoning Province Oral Diseases Translation Medcicne Research Center, Shenyang 110002, China

引用本文:

李改明,刘思雨,战德松,刘蕊,任玲,杨柯,王敬人,王强. 3D打印医用钛合金的抗菌性能和体外生物相容性[J]. 材料研究学报, 2019, 33(2): 117-123.
Gaiming LI, Siyu LIU, Desong ZHAN, Rui LIU, Ling REN, Ke YANG, Jingren WANG, Qiang WANG. Antibacterial Properties and Biocompatibility of SLM-fabricated Medical Titanium Alloys[J]. Chinese Journal of Materials Research, 2019, 33(2): 117-123.

全文: PDF(10477 KB) HTML

摘要:

应用选择性激光熔融技术(SLM)制备出3D打印医用钛合金Ti-6Al-4V和Ti-6Al-4V-5Cu，用平板共培养法研究测定其抗菌性能，用CCK8细胞增殖测定法、鬼笔环肽细胞骨架染色法和Annexin-V/PI流式细胞术研究了这种合金的抗菌性能和对小鼠胚胎成骨前体细胞(MC3T3-E1)的体外生物相容性影响。结果表明，3D打印Ti-6Al-4V-5Cu合金具有较高的抗菌性能，对金黄色葡萄球菌的抗菌率达到(57.03±1.55)%。在CCK8细胞增殖毒性测定、细胞骨架鬼笔环肽染色实验和Annexin-V/PI双标记法流式分析三种研究中Ti-6Al-4V-5Cu表现的优越，具有更好的体外生物相容性。

关键词 ：金属材料, 3D打印, SLM, Ti-6Al-4V-5Cu, 抗菌, 生物相容性

Abstract：

The 3D printing medical titanium alloys Ti-6Al-4V and Ti-6Al-4V-5Cu were prepared by selective laser melting technology (SLM), and their antibacterial properties were assessed by plate co-culture method. The in vitro biocompatibility with the mouse embryonic osteogenic precursor cells (MC3T3-E1) of the prepared alloys was systematically investigated by means of methods of CCK8 cell proliferation assay, phalloidin cytoskeleton staining and Annexin-V/PI flow cytometry. The results show that the 3D printing Ti-6Al-4V-5Cu alloy has high antibacterial property and the antibacterial rate against Staphylococcus aureus is 57.03%. The alloy Ti-6Al-4V-5Cu performed well with better in vitro biocompatibility during the three assessments,namely,CCK8 cell proliferation toxicity assay, cytoskeleton phalloidin staining experiment and Annexin-V/PI double labeling flow analysis.

Key words： metallic materials 3D printing SLM Ti-6Al-4V-5Cu antibacterial biocompatibility

收稿日期: 2018-09-10

ZTFLH:

R318

基金资助:国家重点研发计划(2018YFC1106601);国家自然科学基金(51631009)

作者简介: 李改明，女，1990年生，硕士生

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https://www.cjmr.org/CN/10.11901/1005.3093.2018.548 或 https://www.cjmr.org/CN/Y2019/V33/I2/117

表1 两种钛合金的成分

表2 细胞毒性分级标准和评定结果

图1 三种钛合金对金黄色葡萄球菌的作用

表3 不同种类钛合金对金黄色葡萄球菌的杀菌率(n=4)

图2 CCK-8细胞增值分析测得的MC3T3-E1细胞的OD值

表4 三种钛合金对 MC3T3-E1 细胞的相对增值率

图3 MC3T3-E1 细胞在三种钛合金表面作用下培养4 h和24 h的罗丹明鬼笔环肽骨架染色

图4 MC3T3-E1 细胞在三种钛合金表面培养72 h流式散点图和柱状图统计分析

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