不同铜含量钴铬合金的体外生物相容性

doi:10.11901/1005.3093.2014.791

材料研究学报

2015, Vol. 29

Issue (8): 627-633 DOI: 10.11901/1005.3093.2014.791

本期目录 | 过刊浏览

不同铜含量钴铬合金的体外生物相容性

赵昕^1,^2,^3,⁴,曹阳^1,^2,³,印准^1,^2,³,张光平⁵,任伊宾⁶,战德松^1,^2,^3,⁴(

)

1. 中国医科大学口腔医学院修复二科沈阳 110002
2. 中国医科大学口腔医学院中心实验室沈阳 110002
3. 辽宁省口腔医学研究所沈阳 110002
4. 辽宁省口腔疾病转化医学研究中心沈阳 110002
5. 中国医科大学附属盛京医院口腔科沈阳 110011
6. 中国科学院金属研究所沈阳 110016

In Vitro Biocompatibility of Co-Cr Alloys with Different Content of Copper

Xin ZHAO^1,^2,^3,⁴,Yang CAO^1,^2,³,Zhun YIN^1,^2,³,Guangping ZHANG⁵,Yibin REN⁶,Desong ZHAN^1,^2,^3,^4,^*(

)

1. Department of Prosthetics, School of Stomatology, China Medical University, Shenyang 110002, China
2. Central Laboratory, School of Stomatology, China Medical University, Shenyang 110002, China
3. Liaoning Institute of Dental Research, Shenyang 110002, China
4. Liaoning Province Ord Diseases Translational Medicine Research Center, Shenyang 110002, China
5. Department of Stomatology, Sheng Jing Hospital of China Medical University, Shenyang 110011, China
6. Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China

引用本文:

赵昕,曹阳,印准,张光平,任伊宾,战德松. 不同铜含量钴铬合金的体外生物相容性[J]. 材料研究学报, 2015, 29(8): 627-633.
Xin ZHAO, Yang CAO, Zhun YIN, Guangping ZHANG, Yibin REN, Desong ZHAN. In Vitro Biocompatibility of Co-Cr Alloys with Different Content of Copper[J]. Chinese Journal of Materials Research, 2015, 29(8): 627-633.

全文: PDF(5248 KB) HTML

摘要:

用CCK-8法和Annexin-V/PI双标记法研究了不同铜含量(质量分数, 下同)的钴铬合金和钴铬合金的体外生物相容性(包括L929细胞增殖和凋亡)的影响。结果表明, 4种合金的体外生物相容性按含1%铜的钴铬合金、含2%铜的钴铬合金、钴铬合金、含3%铜的钴铬合金的顺序递减; 其细胞毒性等级都为1级, 具有良好的体外生物相容性。

关键词 ：金属材料, 含铜钴铬合金, 细胞毒性, 细胞增殖, 细胞凋亡, 生物相容性

Abstract：

The in vitro biocompatibility (i.e. the cell proliferation and apoptosis of L929 cells) of 4 cast dental alloys (CoCrMo+1%Cu, CoCrMo+2%Cu, CoCrMo+3%Cu and CoCrMo) were evaluated by CCK-8 and Annexing-V/PI double marking methods to provide a biology reference in the clinical application of prosthodontics. Results show that the in vitro biocompatibility of 4 alloys decreases according to following sequence: CoCrMo+1%Cu, CoCrMo+2%Cu, CoCrMo and CoCrMo+3%Cu. However, the cytotoxicity grades of the 4 alloys are all ranked in level one, implying they are good enough in vitro biocompatibility.

Key words： metallic materials copper-cobalt-chromium alloy cytotoxicity cellproliferation apoptosis biocompatibility

收稿日期: 2014-12-31

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https://www.cjmr.org/CN/10.11901/1005.3093.2014.791 或 https://www.cjmr.org/CN/Y2015/V29/I8/627

表1 实验用合金分组及其主要成分

图1 各组细胞在各时间点的细胞形态

表2 4组合金对L929细胞的相对增殖率和细胞毒性等级

表3 不同培养时间点各组的吸光度值( x ? ±s , n=6)

图3 各组细胞培养48 h后流式细胞仪检测早期凋亡率

表4 各组细胞48 h早期凋亡率( x ? ±s , n=3)

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