基于纳米纤维素的三维组织工程支架多孔结构的调控与表征*

doi:10.11901/1005.3093.2014.272

材料研究学报

2014, Vol. 28

Issue (10): 721-729 DOI: 10.11901/1005.3093.2014.272

本期目录 | 过刊浏览

基于纳米纤维素的三维组织工程支架多孔结构的调控与表征*

唐爱民(

),赵姗,宋建康

华南理工大学制浆造纸国家重点实验室广州 510640

Structure Control and Characterization of 3D Porous Scaffold Based on Cellulose-nanofibers for Tissue Engineering

Aimin TANG(

),Shan ZHAO,Jiankang SONG

State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640

引用本文:

唐爱民,赵姗,宋建康. 基于纳米纤维素的三维组织工程支架多孔结构的调控与表征*[J]. 材料研究学报, 2014, 28(10): 721-729.
Aimin TANG, Shan ZHAO, Jiankang SONG. Structure Control and Characterization of 3D Porous Scaffold Based on Cellulose-nanofibers for Tissue Engineering[J]. Chinese Journal of Materials Research, 2014, 28(10): 721-729.

全文: PDF(10494 KB) HTML

摘要:

本文将纳米纤维素(CNFs)与聚乙烯醇(PVA)混合, 采用冷冻干燥方法制备了纳米纤维素三维多孔组织工程支架。探讨CNFs悬浮液浓度、PVA的相对分子质量、CNFs与PVA的质量比以及冷冻温度对支架孔隙率、孔径分布以及机械性能的影响。结果表明: CNFs/高相对分子质量PVA支架具有梯度分布、内部连通的孔结构, PVA形成大孔的孔壁, 直径100-200 nm的CNFs束则在孔壁上桥接为类似于细胞外基质(ECM)中胶原骨架的网状结构; CNFs浓度为0.5%、CNFs与PVA的质量比为1∶2、冷冻温度为-80℃时, 支架内部微丝丰富且网络结构较好; 支架力学强度主要由PVA提供, 支架的压缩模量随PVA用量的增加而增大, 压缩模量在kPa数量级, 与软骨组织的弹性模量相当, 可通过改变PVA的添加量调节支架材料的力学性能。

关键词 ：复合材料, 纳米纤维素, 聚乙烯醇, 三维多孔, 组织工程支架

Abstract：

Three dimensional porous scaffolds for tissue engineering were fabricated by freeze drying a blend suspension of cellulose-nanofibers (CNFs) with poly (vinyl alcohol) (PVA), while the Influences of the concentration of CNFs suspension, the mass ratio of CNFs to PVA, the relative molecular mass (mass average) of PVA and the frozen temperature on the morphology and mechanical properties of the scaffolds were investigated. The results showed that: the scaffolds made of CNFs with high molecular mass (mass average) PVA displayed morphology of interconnected pores with irregular open pore geometry; PVA formed the wall of the big pores and which were bridged each other by CNFs bundles of 100-200 nm in diameter to form nest-like structure on the surface of the PVA wall throughout the scaffold, which was similar to collagen skeleton in extra cellular matrix (ECM); the optimal parameters for the fabrication of scaffolds with outstanding nest structure and abundant microfilaments are: mass fraction of CNFs to PVA was 1: 2 and the freezing temperature was -80℃. PVA played a key role in providing the mechanical strength of the scaffolds. The compressive modulus of the supports increased with the increasing amount of PVA, and it was equivalent to that of cartilage tissue, a magnitude of kilopascal, and besides, the mechanical properties of the scaffolds can be adjusted by changing the amount of PVA.

Key words： composites cellulose-nanofibers (CNFs) polyvinyl alcohol three-dimensional porous tissue engineering scaffolds

收稿日期: 2014-06-04

基金资助:* 国家重点基础研究发展计划2010CB732206资助项目。

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https://www.cjmr.org/CN/10.11901/1005.3093.2014.272 或 https://www.cjmr.org/CN/Y2014/V28/I10/721

表1 三维多孔组织工程支架的制备条件及编号

图1 纳米纤维素与高相对分子质量PVA质量比为0∶1.5、0.5∶0.5、0.5∶1.5的支架材料的数码照片

图2 1.5 g高相对分子质量PVA分别添加到100 g质量分数0%、0.3%和0.5%的CNFs悬浮液中在-80℃冷冻制备的支架的SEM像

图3 1.5 g低相对分子质量PVA分别添加到100 g质量分数0%、0.3%和0.5%的CNFs悬浮液中在-80℃冷冻制备的支架的SEM像

图4 将0.5 g、1.0 g和1.5 g高相对分子质量PVA分别添加到100 g 0.5%的CNFs悬浮液中在-80℃冷冻制备的支架的SEM像

图5 1.0 g高相对分子质量PVA添加到100 g 0.5%的CNFs悬浮液中分别在-20℃ (a、d、g、j)、-80℃ (b、e、h、k)和液氮 (c、f、i、l)冷冻制备的支架的SEM像

图6 高相对分子质量PVA/CNFs支架材料的压缩模量与PVA添加量的关系

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