纳米纤维素/阳离子聚合物复合三维组织工程支架的性能*

doi:10.11901/1005.3093.2014.293

材料研究学报

2015, Vol. 29

Issue (1): 1-9 DOI: 10.11901/1005.3093.2014.293

本期目录 | 过刊浏览

纳米纤维素/阳离子聚合物复合三维组织工程支架的性能*

唐爱民(

),刘远,赵姗

华南理工大学制浆造纸工程国家重点实验室广州 510640

Performance of 3D Tissue Engineering Scaffolds of Nanocellulose/High Cationic Polymers Composite

Aimin TANG(

),Yuan LIU,Shan ZHAO

State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China

引用本文:

唐爱民,刘远,赵姗. 纳米纤维素/阳离子聚合物复合三维组织工程支架的性能*[J]. 材料研究学报, 2015, 29(1): 1-9.
Aimin TANG, Yuan LIU, Shan ZHAO. Performance of 3D Tissue Engineering Scaffolds of Nanocellulose/High Cationic Polymers Composite[J]. Chinese Journal of Materials Research, 2015, 29(1): 1-9.

全文: PDF(8473 KB) HTML

摘要:

将纳米纤维素分别与高阳离子度的丙烯酸类、乙烯胺类聚合物复合, 制备出三维多孔组织工程支架并通过SEM观察了支架的形貌结构, 研究了高阳离子度的丙烯酸类、乙烯胺类聚合物的相对分子质量和用量对支架孔隙结构的影响; 用图像处理软件对采集的支架截面SEM图进行处理, 通过统计计算获得支架孔隙率数据, 建立了一种基于SEM图像快速测定支架孔隙率的新方法; 最后测定了支架的保水值。结果表明: 本文制备的纳米纤维素三维组织工程支架的孔隙率均大于90％。用图像处理得到的孔隙率与用液体置换法测得的孔隙率数值接近, 误差低于5％, 表明用图像处理方法测定的孔隙率可靠。纳米纤维素三维组织工程支架有很高的保水值(>200％), 调整阳离子聚合物的种类和用量可调控其孔隙率和保水值, 得到适合组织细胞培养的纳米纤维素三维组织工程支架。

关键词 ：复合材料, 纳米纤维素, 组织工程支架, 图像处理

Abstract：

Three-dimensional (3D) tissue engineering scaffolds were prepared by compounding nanocellulose with polyacrylic cationic polymer and polyethylene amine cationic polymer respectively. The structural morphology of the scaffolds was characterized by scanning electron microscopy (SEM).The influence of the relative molecular mass and dosages of the polymers on the pore structure of the scaffolds was investigated, while a new method for fast measuring the porosity of the scaffolds was established based on SEM image processing. The water retention value (WRV) of the scaffolds was also measured. Results show that the porosity of all the nanocellulose 3D tissue engineering scaffolds is larger than 90％. The porosity value obtained by the new image processing method is close to that measured according to Archimedes principle with a difference less than 5%, which indicated that this method was reliable. All the 3D scaffolds have high WRV (>200％). Both the porosity and WRV of the 3D scaffolds can be adjusted by varying the species and dosage of polymers. Therewith the nanocellulose 3D tissue engineering scaffolds may optionally be prepared to meet the requirement for tissue and cell culture.

Key words： composites nanocellulose tissue engineering scaffold image processing

收稿日期: 2014-06-18

基金资助:* 国家重点基础研究发展计划2010CB732206资助项目。

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https://www.cjmr.org/CN/10.11901/1005.3093.2014.293 或 https://www.cjmr.org/CN/Y2015/V29/I1/1

图1 纳米纤维素的AFM像

图2 纳米纤维素与聚合物A复合支架的SEM像

图3 纳米纤维素与聚合物B复合支架的SEM像

图4 液体置换法测定的纳米纤维素分别与聚合物A、B复合支架的孔隙率

表1 纳米纤维素/聚合物A复合支架孔隙率的图像处理法和液体置换法测试数据比较

表2 纳米纤维素/聚合物B复合支架孔隙率的图像处理法和液体置换法测试数据比较

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