金属有机框架固定化漆酶的制备及其对邻苯二酚的降解

doi:10.11901/1005.3093.2021.244

材料研究学报

2022, Vol. 36

Issue (10): 793-800 DOI: 10.11901/1005.3093.2021.244

研究论文

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金属有机框架固定化漆酶的制备及其对邻苯二酚的降解

侯语桐¹, 李鑫¹, 李大伟², 魏取福¹(

)

^1.江南大学生态纺织教育部重点实验室无锡 214122
^2.南通大学纺织服装学院南通 226019

Preparation of Immobilized Laccase onto Framework-like Material of Metal-organic Compound and Its Degradation for Catechol

HOU Yutong¹, LI Xin¹, LI Dawei², WEI Qufu¹(

)

^1.Key Laboratory of Eco-Textiles, Jiangnan University, Wuxi 214122, China
^2.College of Textile and Clothing, Nantong University, Nantong 226019, China

引用本文:

侯语桐, 李鑫, 李大伟, 魏取福. 金属有机框架固定化漆酶的制备及其对邻苯二酚的降解[J]. 材料研究学报, 2022, 36(10): 793-800.
Yutong HOU, Xin LI, Dawei LI, Qufu WEI. Preparation of Immobilized Laccase onto Framework-like Material of Metal-organic Compound and Its Degradation for Catechol[J]. Chinese Journal of Materials Research, 2022, 36(10): 793-800.

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摘要:

以金属有机框架材料(ZIF-90)为固定载体,采用内部封装和表面物理吸附两种方法固定漆酶(LAC),分别制备出LAC@ZIF-90和LAC-ZIF-90复合材料,使用扫描电镜(SEM)、激光共聚焦显微镜(CLSM)、X射线衍射(XRD)、傅里叶红外变换光谱(FT-IR)以及荧光分析等手段分析漆酶固定前后的结构和性能,对比分析了游离漆酶和固定漆酶的使用稳定性,根据高效液相色谱(HPLC)研究了固定化漆酶对邻苯二酚的降解性能。结果表明：漆酶分子固定在ZIF-90的表面和内部,分子结构稳定。在最适当的pH值条件下,LAC-ZIF-90和LAC@ZIF-90震荡6 h对邻苯二酚的去除率分别为97%和19.4%。LAC-ZIF-90和LAC@ZIF-90经过5次重复使用后仍具有较高的活力,LAC-ZIF-90重复5次后对邻苯二酚的去除率仍高于78%。

关键词 ：复合材料, 金属有机框架材料, 漆酶, 固定, 邻苯二酚, 降解

Abstract：

Laccase (LAC) was immobilized onto a framework-like material of metal-organic compound (ZIF-90) by means of internal encapsulation and surface physical-adsorption, herewith, two composites of LAC@ZIF-90 and LAC-ZIF-90 were prepared respectively. The structure, property, stability and degradation performance for catechol of the free- and immobilized-laccase were comparatively characterized by means of scanning electron microscopy (SEM), laser confocal electron microscopy (CLSM), X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), fluorescence analysis and high performance liquid chromatography (HPLC) etc. The results show that: laccase molecules were fixed on the surface and inside of ZIF-90, and the molecular structure was stable before and after being fixed; the removal rates for catechol by lac-ZIF-90 and LAC@ZIF-90 were 97% and 19.4%, respectively for the solution with proper pH value, whilst by vibrating for 6 h; the lac-ZIF-90 and LAC@ZIF-90 still showed high vitality after repeatedly used for 5 times; it is especially worthy that the removal rate of catechol was higher than 78% even after repeatedly used for 5 times for the lac-ZIF-90.

Key words： composits metal organic framework materials laccase immobilization catechol degradation

收稿日期: 2021-04-15

ZTFLH:

TB332

基金资助:江苏省自然科学基金(BK20180628);中央高校基本科研业务费专项资金(JUSRP51907A);江苏高校优势学科建设工程资助项目(苏政办发［2014］37号)

作者简介: 侯语桐,女,1996年生,硕士生

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https://www.cjmr.org/CN/10.11901/1005.3093.2021.244 或 https://www.cjmr.org/CN/Y2022/V36/I10/793

图1 LAC@ZIF-90和LAC-ZIF-90的合成示意图

图2 ZIF-90、LAC@ZIF-90和LAC-ZIF-90的SEM照片

图3 CLSM显示LAC-ZIF-90(a,b,c)和LAC@ZIF-90(d,e,f)的荧光、明场和重叠图像

图4 ZIF-90、LAC@ZIF-90和LAC-ZIF-90的XRD谱

图5 ZIF-90、LAC@ZIF-90、LAC-ZIF-90和游离LAC的FTIR谱

图6 ZIF-90、LAC@ZIF-90、LAC-ZIF-90和游离LAC的荧光光谱

表1 LAC、LAC-ZIF-90和LAC@ZIF-90的孔隙特征

图7 pH值对固定漆酶活性的影响

图8 温度对固定漆酶活性的影响

图9 游离漆酶、固定漆酶以及ZIF-90对邻苯二酚的去除性能

图10 固定漆酶的重复使用性

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