The fatigue behavior of A319 cast aluminum alloy was investigated in terms of its cycle stress response characters and fatigue life under a 0.2% strain amplitude with several loading paths, such as uniaxial, proportional and non-proportional ones. While the fracture characters of failed specimen and the cracking modes of Si particles were also investigated. Under the condition of same equivalent stress, the effectiveness of the three loading paths on the hardening of the alloy in terms of the degree and rate of hardening may be ranked in an order of high to low as follows: non-proportional > proportional > uniaxial, corresponding with the fatigue life exactly. The fractography of the alloy presented two major cracks with the herringbone pattern under the proportional loading. Additionally, the crack initiation site was gradually blurred when the loading path changing from uniaxial, to proportional and then to nonproportional ones, in the meanwhile the size of the crack initiation site and propagation region also decreased. The crack surface basically paralleled to the loading direction under uneasily loading, however, multi-cracks with different directions occurred under multi-axial loading.