In Vitro Biocompatibility of Co-Cr Alloys with Different Content of Copper

doi:10.11901/1005.3093.2014.791

Chinese Journal of Materials Research

2015, Vol. 29

Issue (8): 627-633 DOI: 10.11901/1005.3093.2014.791

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In Vitro Biocompatibility of Co-Cr Alloys with Different Content of Copper

Xin ZHAO^1,^2,^3,⁴,Yang CAO^1,^2,³,Zhun YIN^1,^2,³,Guangping ZHANG⁵,Yibin REN⁶,Desong ZHAN^1,^2,^3,^4,^*(

)

1. Department of Prosthetics, School of Stomatology, China Medical University, Shenyang 110002, China
2. Central Laboratory, School of Stomatology, China Medical University, Shenyang 110002, China
3. Liaoning Institute of Dental Research, Shenyang 110002, China
4. Liaoning Province Ord Diseases Translational Medicine Research Center, Shenyang 110002, China
5. Department of Stomatology, Sheng Jing Hospital of China Medical University, Shenyang 110011, China
6. Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China

Cite this article:

Xin ZHAO,Yang CAO,Zhun YIN,Guangping ZHANG,Yibin REN,Desong ZHAN. In Vitro Biocompatibility of Co-Cr Alloys with Different Content of Copper. Chinese Journal of Materials Research, 2015, 29(8): 627-633.

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Abstract

The in vitro biocompatibility (i.e. the cell proliferation and apoptosis of L929 cells) of 4 cast dental alloys (CoCrMo+1%Cu, CoCrMo+2%Cu, CoCrMo+3%Cu and CoCrMo) were evaluated by CCK-8 and Annexing-V/PI double marking methods to provide a biology reference in the clinical application of prosthodontics. Results show that the in vitro biocompatibility of 4 alloys decreases according to following sequence: CoCrMo+1%Cu, CoCrMo+2%Cu, CoCrMo and CoCrMo+3%Cu. However, the cytotoxicity grades of the 4 alloys are all ranked in level one, implying they are good enough in vitro biocompatibility.

Key words: metallic materials copper-cobalt-chromium alloy cytotoxicity cellproliferation apoptosis biocompatibility

Received: 31 December 2014

About author: *To whom correspondence should be addressed, Tel: (024)22894863, E-mail: zhandesong@126.com

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	Xin ZHAO
	Yang CAO
	Zhun YIN
	Guangping ZHANG
	Yibin REN
	Desong ZHAN

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https://www.cjmr.org/EN/10.11901/1005.3093.2014.791 OR https://www.cjmr.org/EN/Y2015/V29/I8/627

Table 1 Main compositions of experimental alloys (mass fraction,%)

Fig.1 Cell morphology at each time points (A) CoCrMo group; (B) CoCrMo+1%Cu group; (C) CoCrMo+2%Cu group; (D) CoCrMo+3%Cu group; (E) negative control group; (F) positive control group

Table 2 The cell RGR and cytotoxicity grades of 4 kinds of alloys to L929

Table 3 Absorption value at different culture time points( x ? ±s , n=6)

Fig.3 Results of flow cytometry about early apoptosis after 48 h. (a) CoCrMo group; (b) CoCrMo+1%Cu group; (c) CoCrMo+2%Cu group; (d) CoCrMo+3%Cu group; (e) negative control group

Table 4 Table 4 Early apoptosis rate of each group at 48 h( x ? ±s , n=3)

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