Antibacterial Properties and Biocompatibility of SLM-fabricated Medical Titanium Alloys

doi:10.11901/1005.3093.2018.548

Chinese Journal of Materials Research

2019, Vol. 33

Issue (2): 117-123 DOI: 10.11901/1005.3093.2018.548

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Antibacterial Properties and Biocompatibility of SLM-fabricated Medical Titanium Alloys

Gaiming LI^1,^2,^4,⁵,Siyu LIU^1,^2,^4,⁵,Desong ZHAN^1,^2,^4,⁵(

),Rui LIU³,Ling REN³,Ke YANG³,Jingren WANG^1,^2,^4,⁵,Qiang WANG^1,^2,^4,⁵

1. Department of Dental Material, School of Stomatology, China Medical University, Shenyang 110002, China
2. Liaoning Institute of Dental Research, Shenyang 110002, China
3. Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China
4. Liaoning Province Oral Diseases Key Laboratory, Shenyang 110002, China
5. Liaoning Province Oral Diseases Translation Medcicne Research Center, Shenyang 110002, China

Cite this article:

Gaiming LI,Siyu LIU,Desong ZHAN,Rui LIU,Ling REN,Ke YANG,Jingren WANG,Qiang WANG. Antibacterial Properties and Biocompatibility of SLM-fabricated Medical Titanium Alloys. Chinese Journal of Materials Research, 2019, 33(2): 117-123.

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Abstract

The 3D printing medical titanium alloys Ti-6Al-4V and Ti-6Al-4V-5Cu were prepared by selective laser melting technology (SLM), and their antibacterial properties were assessed by plate co-culture method. The in vitro biocompatibility with the mouse embryonic osteogenic precursor cells (MC3T3-E1) of the prepared alloys was systematically investigated by means of methods of CCK8 cell proliferation assay, phalloidin cytoskeleton staining and Annexin-V/PI flow cytometry. The results show that the 3D printing Ti-6Al-4V-5Cu alloy has high antibacterial property and the antibacterial rate against Staphylococcus aureus is 57.03%. The alloy Ti-6Al-4V-5Cu performed well with better in vitro biocompatibility during the three assessments,namely,CCK8 cell proliferation toxicity assay, cytoskeleton phalloidin staining experiment and Annexin-V/PI double labeling flow analysis.

Key words: metallic materials 3D printing SLM Ti-6Al-4V-5Cu antibacterial biocompatibility

Received: 10 September 2018

ZTFLH:

R318

Fund: Supported by National Key Research and Development Plan(2018YFC1106601);National Natural Science Foundation of China(51631009)

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	Gaiming LI
	Siyu LIU
	Desong ZHAN
	Rui LIU
	Ling REN
	Ke YANG
	Jingren WANG
	Qiang WANG

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https://www.cjmr.org/EN/10.11901/1005.3093.2018.548 OR https://www.cjmr.org/EN/Y2019/V33/I2/117

Table 1 Composition of two titanium alloys (%, mass fraction)

Table 2 Classification standard of cytotoxicity

Fig.1 Antimicrobial effects of three Titanium Alloys against staphylococcus aureus (a, b) TC4-5Cu group (staphylococcus aureus); (c, d) TC4-c group (staphylococcus aureus); (e, f) tc4 group (staphylococcus aureus)

Table 3 Treatment of antibacterial rate of staphylococcus aureus with different kinds of titanium alloys (n=4)

Fig.2 OD values of MC3T3-E1 cells measured by CCK-8 cell proliferation assay

Table 4 Relative growth rate (RGR) of three titanium alloys on MC3T3-E1 cells

Fig.3 Co-culture of MC3T3-E1 cells on the surface of three titanium alloys for 4 h and 24 h rhodamine phalloidin backbone staining. 4 h: (a1) TC4-5Cu group; (b1) TC4 group; (c1) tc4 group; 24 h: (a2) TC4-5Cu group; (b2) TC4 group; (c2) tc4 group

Fig.4 Co-culture of MC3T3-E1 cells on titanium alloys for 72 h flow scatter and bar graph statistical analysis (a) TC4-5Cu group; (b) TC4 group; (c) tc4 group; (d) normal cell viability; (e) apotoic cell viability

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