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Chinese Journal of Materials Research  2017, Vol. 31 Issue (8): 612-618    DOI: 10.11901/1005.3093.2016.525
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Preparation and Properties of Composite Films of Silk Fibroin/Carboxymethyl Chitosan
Shui XU1,2(), Yan ZHANG1,2, Baodong GAO1,2, Zhao ZHAO1,2, Guotao CHENG1,2, Yong ZHU1,2
1 College of Biotechnology, Laboratory of Fiber Materials of Southwest University,Chongqing 400715, China
2 Research Center of Silk Fiber Materials Engineering of Chongqing, Chongqing 400715, China
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Shui XU, Yan ZHANG, Baodong GAO, Zhao ZHAO, Guotao CHENG, Yong ZHU. Preparation and Properties of Composite Films of Silk Fibroin/Carboxymethyl Chitosan. Chinese Journal of Materials Research, 2017, 31(8): 612-618.

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Abstract  

Composite films of regenerated silk fibroin/carboxymethyl chitosan were successfully prepared by quadratic freezing-drying method with glutaraldehyde as cross-linking agents, and then of which the physicochemical performance was characterized. The results show that the composite materials show a three-dimensional porous structure with uniform pore distribution; while there exists intermolecular hydrogen bonds between the silk fibroin and the carboxymethyl chitosan, thus they are compatible with each other; Among others, the prepared composite presents the best mechanical property and good thermal stability when the mass ratio of the regenerated silk fibroin to the carboxymethyl chitosan was 1:1 and the glutaraldehyde content was 0.20%. The composite material has also good biodegradability and biocompatibility.
Key words:  composite      biomaterials      composite membrane material      lyophilization      regenerated silk fibroin      carboxymethyl chitosan      biocompatibility     
Received:  08 September 2016     
ZTFLH:  TQ341.5  
Fund: Supported by Research Center of Silk Fiber Materials Engineering of Chongqing (No.SILKGCZX009) and Jiangsu Key Laboratory of Silk Engineering, Soochow University (No.KJS1508)
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Shui XU
Yan ZHANG
Baodong GAO
Zhao ZHAO
Guotao CHENG
Yong ZHU

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https://www.cjmr.org/EN/10.11901/1005.3093.2016.525     OR     https://www.cjmr.org/EN/Y2017/V31/I8/612

No. SF/CMCS(g/g) GA(%)
1 3:1 0.20
2 2:1 0.20
3 1:1 0.20
4 1:2 0.20
5 1:3 0.20
6 1:1 0.05
7 1:1 0.10
8 1:1 0.15
9 1:1 0
Table 1  Preparation process of regenerated fibroin/carboxymethyl chitosan composites
SF:CMCS Dissolution
rate		 Swelling
capacity		 Water
content rate
3:1 56.55 279.03 24.30
2:1 58.66 266.31 32.54
1:1 59.41 391.87 32.89
1:2 77.37 591.96 34.33
1:3 80.37 447.51 33.78
Table 2  Dissolution rate, swelling capacity and water content rate of different composites (%)
GA Dissolution
rate		 Swelling
capacity		 Water
content rate
0 70.00 — 42.47
0.05 64.38 914.86 37.21
0.10 64.35 699.39 33.63
0.15 57.91 643.01 31.91
0.20 59.41 391.87 32.89
Table 3  Dissolution rate, swelling capacity and water content rate of different glutaraldehyde content (%)
Fig.1  Mechanicalproperies of different composites
Fig.2  Mechanicalproperies of different composites with different glutaraldehyde content
Fig.3  Thermogravimetric curves of different SF/CMCS composites
Fig.4  Thermogravimetric curves of SF/CMCS composites with different glutaraldehyde content
Fig.5  FTIR spectra of SF, CMCS and SF/CMCS composites
Fig.6  FTIR spectra of crosslink and uncrosslink SF/CMCS composites
Fig.7  SEM images of regenerated fibroin/arboxymethyl chitosan composites A (a)、B (b)、C (c) refer SF/CMCS 2/1, 1/1, 1/2
Fig.8  The histological observation of composites implanted subcutaneous (a, b, c) the histological observation of composites 1 after operation 1, 2, 4 weeks, (d, e, f) the histological observation of composites 2 after operation 1, 2, 4 weeks
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