输尿管支架表面化学接枝镀铜涂层及其性能

doi:10.11901/1005.3093.2021.323

材料研究学报

2022, Vol. 36

Issue (10): 721-729 DOI: 10.11901/1005.3093.2021.323

研究论文

本期目录 | 过刊浏览

输尿管支架表面化学接枝镀铜涂层及其性能

李建中¹^,², 朱博轩¹, 王振宇², 赵静¹(

), 范连慧², 杨柯¹

^1.中国科学院金属研究所沈阳 110016
^2.中国人民解放军北部战区总医院沈阳 110083

Preparation and Properties of Copper-carrying Polydopamine Coating on Ureteral Stent

LI Jianzhong¹^,², ZHU Boxuan¹, WANG Zhenyu², ZHAO Jing¹(

), FAN Lianhui², YANG Ke¹

^1.Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China
^2.General Hospital of Northern Theater Command, PLA, Shenyang 110083, China

引用本文:

李建中, 朱博轩, 王振宇, 赵静, 范连慧, 杨柯. 输尿管支架表面化学接枝镀铜涂层及其性能[J]. 材料研究学报, 2022, 36(10): 721-729.
Jianzhong LI, Boxuan ZHU, Zhenyu WANG, Jing ZHAO, Lianhui FAN, Ke YANG. Preparation and Properties of Copper-carrying Polydopamine Coating on Ureteral Stent[J]. Chinese Journal of Materials Research, 2022, 36(10): 721-729.

全文: PDF(3303 KB) HTML

摘要:

采用聚多巴胺接枝化学镀铜方法在316L不锈钢表面制备载铜聚多巴胺涂层,使用扫描电镜、原子力显微镜、X射线光电子能谱、电感耦合等离子体质谱仪等手段表征其表面形貌、成分和铜离子释放量并将其与细菌和细胞共培养,研究了涂层的抗感染、抗结石性能和生物相容性。结果表明,载铜涂层的厚度为27 nm,分布均匀,其中的铜以Cu、CuO和Cu₂O的形式存在。将涂层在人工尿液中浸泡14 d,铜离子每天的释放量接近。将涂层分别与大肠杆菌和金黄色葡萄球菌培养24 h后,抗菌率为96.2%和95.9%。在金黄色葡萄球菌悬液中浸泡30 d后在涂层表面沉积的钙离子和镁离子含量分别为48.7 mg/L和235.3 mg/L,明显低于对照组。细胞增殖实验的结果表明,这种涂层无细胞毒性。

关键词 ：材料表面与界面, 功能涂层, 化学接枝镀铜法, 输尿管支架, 抗结石, 生物相容性

Abstract：

Bacteria adhesion and encrustation formation on the surface of ureteral stents have been common complications clinically. Herewith, the Cu grafting polydopamine coating was prepared on 316L stainless steel via polydopamine graft agent assisted electroless Cu plating method, in order to create a coating with performance of anti-infection, anti-stone formation and good biocompatibility for the ureteral stent surface. While the surface morphology, composition and copper ions release of the coatings were assessed by means of scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy and inductively coupled plasma mass spectrometry. Samples were incubated with bacteria and cells respectively, so that to examine the antibacterial ability, encrustation resistance and biocompatibility of coatings. The results show that the coating is evenly distributed. The copper in the coating consists of Cu, CuO and Cu₂O and its thickness is 27.0 nm. The content of releasing copper for each day remains nearly constant after immersion in artificial urine. The antibacterial rates against Escherichia coli and Staphylococcus aureus are 96.2% and 95.9% after incubating for 24 hours, respectively. The contents of calcium and magnesium of samples, which are deposited in the human urine coupled with Staphylococcus aureus for 30 days, are 48.7 mg/L and 235.3 mg/L, respectively, which is significantly lower than that of the control group. Cells proliferation assay shows no cytotoxicity.

Key words： surface and interface in the materials functional coating chemical grafting copper ureteral stent anti-encrustation biocompatibility

收稿日期: 2021-05-24

ZTFLH:

O647.9

基金资助:国家重点研发计划(2018YFC1105504);辽宁省博士科研启动基金计划(2019-BS-255);辽宁省科学技术计划(2020JH2/10300159)

作者简介: 李建中,男,1985年生,博士生

	服务

	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	李建中
	朱博轩
	王振宇
	赵静
	范连慧
	杨柯
44.8K

链接本文:

https://www.cjmr.org/CN/10.11901/1005.3093.2021.323 或 https://www.cjmr.org/CN/Y2022/V36/I10/721

表1 人工尿液的成分[23]

图1 316L不锈钢和载铜聚多巴胺涂层样品表面的形貌

图2 载铜聚多巴胺涂层的面扫图

图3 316L不锈钢、聚多巴胺涂层和载铜聚多巴胺涂层样品的AFM形貌

图4 用椭圆偏振光谱仪检测样品的膜厚

图5 载铜聚多巴胺涂层的XPS分析结果

表2 溅射不同时间的载铜聚多巴胺涂层中Cu、CuO和Cu2O的含量

图6 不同样品在人工尿液中浸泡后每天释放的铜离子含量

图7 不同材料与细菌培养24 h后的活细菌数量和抗菌率

图8 材料与细菌浸泡培养30 d后活细菌的数量和抗菌率

图9 不同材料在尿液中沉积30 d后表面结石中钙、镁离子的含量

图10 UECs与不同材料浸提液培养不同时间后的相对增殖率

图11 多巴胺聚合反应式[26]

1	Garg A, Mishra K G, Bharti P K, et al. Observation of double J stent bacterial colonisation and its correlation with bacteriuria frequency [J]. J. Evol. Med. Dent. Sci., 2017, 6: 5861
2	Szell T, Dressler F F, Goelz H, et al. In vitro effects of a novel coating agent on bacterial biofilm development on ureteral stents [J]. J. Endourol., 2019, 33: 225 doi: 10.1089/end.2018.0616
3	Lombardo R, Tubaro A, Nunzio C D. Ureteral stent encrustation: epidemiology, pathophysiology, management and current technology [J]. J. Urol., 2021, 205: 68 doi: 10.1097/JU.0000000000001343
4	Oderda M, Lacquaniti S, Fasolis G. Allium stent for the treatment of a malignant ureteral stenosis: a paradigmatic case [J]. Urologia J., 2018, 85: 87 doi: 10.5301/uj.5000249
5	Choi J, Chung K J, Choo S H, et al. Long-term outcomes of two types of metal stent for chronic benign ureteral strictures [J]. BMC Urol., 2019, 19: 34 doi: 10.1186/s12894-019-0465-5 pmid: 31060531
6	Rebl H, Renner J, Kram W, et al. Prevention of encrustation on ureteral stents: which surface parameters provide guidance for the development of novel stent materials? [J]. Polymers, 2020, 12: 558 doi: 10.3390/polym12030558
7	Roy R, Tiwari M, Donelli G, et al. Strategies for combating bacterial biofilms: a focus on anti-biofilm agents and their mechanisms of action [J]. Virulence, 2018, 9: 522 doi: 10.1080/21505594.2017.1313372 pmid: 28362216
8	Mosayyebi A, Vijayakumar A, Yue Y Q, et al. Engineering solutions to ureteral stents: material, coating and design [J]. Cent. Eur. J. Urol., 2017, 70: 270
9	Riedl C R, Witkowski M, Plas E, et al. Heparin coating reduces encrustation of ureteral stents: a preliminary report [J]. Int. J. Antimicrob. Agents, 2002, 19: 507 doi: 10.1016/S0924-8579(02)00097-3
10	Chew B H, Davoudi H, Li J M, et al. An in vivo porcine evaluation of the safety, bioavailability, and tissue penetration of a ketorolac drug-eluting ureteral stent designed to improve comfort [J]. J. Endourol., 2010, 24: 1023 doi: 10.1089/end.2009.0523 pmid: 20367085
11	Krambeck A E, Walsh R S, Denstedt J D, et al. A novel drug eluting ureteral stent: a prospective, randomized, multicenter clinical trial to evaluate the safety and effectiveness of a ketorolac loaded ureteral stent [J]. J. Urol., 2010, 183: 1037 doi: 10.1016/j.juro.2009.11.035 pmid: 20092835
12	Felício M R, Silveira G G O S, Oshiro K G N, et al. Polyalanine peptide variations may have different mechanisms of action against multidrug-resistant bacterial pathogens [J]. J. Antimicrob. Chemother., 2021, 76: 1174 doi: 10.1093/jac/dkaa560 pmid: 33501992
13	Mishra B, Basu A, Chua R R Y, et al. Site specific immobilization of a potent antimicrobial peptide onto silicone catheters: evaluation against urinary tract infection pathogens [J]. J. Mater. Chem., 2014, 2B: 1706
14	Gultekinoglu M, Sarisozen Y T, Erdogdu C, et al. Designing of dynamic polyethyleneimine (PEI) brushes on polyurethane (PU) ureteral stents to prevent infections [J]. Acta Biomater., 2015, 21: 44 doi: 10.1016/j.actbio.2015.03.037 pmid: 25848724
15	Leigh B L, Cheng E, Xu L J, et al. Antifouling photograftable zwitterionic coatings on PDMS substrates [J]. Langmuir, 2019, 35: 1100 doi: 10.1021/acs.langmuir.8b00838 pmid: 29983076
16	Wang Y X, Sun Y L, Gu Y H, et al. Articular cartilage-inspired surface functionalization for enhanced lubrication [J]. Adv. Mater. Interfaces, 2019, 6:1900180 doi: 10.1002/admi.201900180
17	Ren L, Xu L, Feng J W, et al. In vitro study of role of trace amount of Cu release from Cu-bearing stainless steel targeting for reduction of in-stent restenosis [J]. J. Mater. Sci. Mater. Med., 2012, 23: 1235 doi: 10.1007/s10856-012-4584-8
18	Ren L, Xu X H, Liu H, et al. Biocompatibility and Cu ions release kinetics of copper-bearing titanium alloys [J]. J. Mater. Sci. Technol., 2021, 95: 237 doi: 10.1016/j.jmst.2021.03.074
19	Jin S J, Qi X, Wang T M, et al. In vitro study of stimulation effect on endothelialization by a copper bearing cobalt alloy [J]. J. Biomed. Mater. Res., 2018, 106A: 561
20	Zhao J, Ren L, Zhang B C, et al. In vitro study on infectious ureteral encrustation resistance of Cu-bearing stainless steel [J]. J. Mater. Sci. Technol., 2017, 33: 1604 doi: 10.1016/j.jmst.2017.03.025
21	Zhao J, Cao Z Q, Lin H, et al. In vivo research on Cu-bearing ureteral stent [J]. J. Mater. Sci. Mater. Med., 2019, 30: 83 doi: 10.1007/s10856-019-6285-z
22	Jin Y Y, Zhu Z Q, Liang L, et al. A facile heparin/carboxymethyl chitosan coating mediated by polydopamine on implants for hemocompatibility and antibacterial properties [J]. Appl. Surf. Sci., 2020, 528: 146539 doi: 10.1016/j.apsusc.2020.146539
23	Haddad L. Synthetic urine and method of making same[P]. US Pat, 7109035, 2006
24	Shen F Y. Construction of copper mediated in situ nitric oxide-generating coating for cardiovascular interventional devices [D]. Chengdu: Southwest Jiaotong University, 2016
24	申芳瑜. 用于血管介入器械表面改性的铜介导原位催化产生一氧化氮涂层的构建 [D]. 成都: 西南交通大学, 2016
25	Xu C H, Tian M, Liu L, et al. Fabrication and properties of silverized glass fiber by dopamine functionalization and electroless plating [J]. J. Electrochem. Soc., 2012, 159: D217 doi: 10.1149/2.056204jes
26	Wang L R, Guan H Y, Chen S S, et al. Preparation and antibacterial function of an Cu-bearing chitosan coating on silicone rubber surface [J]. Chin. J. Mater. Res., 2020, 34: 575 doi: 10.11901/1005.3093.2020.047
26	王立蓉, 关宏宇, 陈姗姗等. 硅橡胶表面壳聚糖载铜凝胶涂层的制备及其抗菌功能 [J]. 材料研究学报, 2020, 34: 575 doi: 10.11901/1005.3093.2020.047
27	Shao H. Construction of multilyered composites from poly (dopamine) modified carbon nanotubes and the interactions with cells [D]. Guangzhou: Jinan University, 2017
27	邵晗. 聚多巴胺修饰碳纳米管静电叠层复合膜的制备及细胞作用研究 [D]. 广州: 暨南大学, 2017
28	Nandakumar R, Santo C E, Madayiputhiya N, et al. Quantitative proteomic profiling of the Escherichia coli response to metallic copper surfaces [J]. Biometals, 2011, 24: 429 doi: 10.1007/s10534-011-9434-5 pmid: 21384090
29	Zanzen U, Bovenkamp-Langlois L, Klysubun W, et al. The interaction of copper ions with Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli: an X-ray absorption near-edge structure (XANES) spectroscopy study [J]. Arch. Microbiol., 2018, 200: 401 doi: 10.1007/s00203-017-1454-2
30	Iribarnegaray V, Navarro N, Robino L, et al. Magnesium-doped zinc oxide nanoparticles alter biofilm formation of Proteus mirabilis [J]. Nanomedicine, 2019, 14: 1551 doi: 10.2217/nnm-2018-0420
31	Multanen M, Talja M, Hallanvuo S, et al. Bacterial adherence to silver nitrate coated poly-L-lactic acid urological stents in vitro [J]. Urol. Res., 2000, 28: 327 pmid: 11127712
32	Milo S, Hathaway H, Nzakizwanayo J, et al. Prevention of encrustation and blockage of urinary catheters by Proteus mirabilis via pH-triggered release of bacteriophage [J]. J. Mater. Chem., 2017, 5B: 5403
33	Donlan R M, Costerton J W. Biofilms: survival mechanisms of clinically relevant microorganisms [J]. Clin. Microbiol. Rev., 2002, 15: 167 doi: 10.1128/CMR.15.2.167-193.2002 pmid: 11932229
34	Stickler D J, Jones G L, Russell A D, et al. Control of encrustation and blockage of Foley catheters [J]. Lancet, 2003, 361: 1435 pmid: 12727400

[1]	陆益敏, 马丽芳, 王海, 奚琳, 徐曼曼, 杨春来. 脉冲激光沉积技术生长铜材碳基保护膜[J]. 材料研究学报, 2023, 37(9): 706-712.
[2]	王乾, 蒲磊, 贾彩霞, 李志歆, 李俊. 碳纤维/环氧复合材料界面改性的不均匀性[J]. 材料研究学报, 2023, 37(9): 668-674.
[3]	冯叶, 陈志勇, 姜肃猛, 宫骏, 单以银, 刘建荣, 王清江. 一种NiCrAlSiY涂层对Ti65钛合金板材循环氧化和室温力学性能的影响[J]. 材料研究学报, 2023, 37(7): 523-534.
[4]	闫春良, 郭鹏, 周靖远, 汪爱英. Cu掺杂非晶碳薄膜的电学性能及其载流子输运行为[J]. 材料研究学报, 2023, 37(10): 747-758.
[5]	陈开旺, 张鹏林, 李树旺, 牛显明, 胡春莲. 莫来石粉末化学镀镍和涂层的高温摩擦学性能[J]. 材料研究学报, 2023, 37(1): 39-46.
[6]	单位摇, 王永利, 李静, 熊良银, 杜晓明, 刘实. 锆合金表面Cr基涂层的耐高温氧化性能[J]. 材料研究学报, 2022, 36(9): 699-705.
[7]	程红杰, 刘黄娟, 姜婷, 王法军, 李文. 近红外反射超疏水黄色涂层的制备和性能[J]. 材料研究学报, 2022, 36(9): 687-698.
[8]	张红亮, 赵国庆, 欧军飞, Amirfazli Alidad. 基于聚多巴胺的超疏水棉织物的一锅法制备及其油水分离性能[J]. 材料研究学报, 2022, 36(2): 114-122.
[9]	崔丽, 孙丽丽, 郭鹏, 马鑫, 王舒远, 汪爱英. 沉积时间对聚醚醚酮表面类金刚石薄膜的结构和性能的影响[J]. 材料研究学报, 2022, 36(11): 801-810.
[10]	李蕊, 王浩, 张天刚, 牛伟. Ti811合金表面激光熔覆Ti₂Ni+TiC+Al₂O₃+Cr_xS_y复合涂层的组织和性能[J]. 材料研究学报, 2022, 36(1): 62-72.
[11]	李修贤, 邱万奇, 焦东玲, 钟喜春, 刘仲武. α籽晶促进低温反应溅射沉积α-Al₂O₃薄膜[J]. 材料研究学报, 2022, 36(1): 8-12.
[12]	范金辉, 李鹏飞, 梁晓军, 梁建平, 徐长征, 蒋力, 叶祥熙, 李志军. 镍-不锈钢复合板轧制过程中界面的结合机制[J]. 材料研究学报, 2021, 35(7): 493-500.
[13]	卢壹梁, 杜瑶, 王成, 辛丽, 朱圣龙, 王福会. 纳米Al₂O₃和TiO₂改性有机硅涂层对304不锈钢高温氧化行为的影响[J]. 材料研究学报, 2021, 35(6): 458-466.
[14]	张会臣, 漆雪莲. 跑合过程引发钛合金水基润滑的超低摩擦特性[J]. 材料研究学报, 2021, 35(5): 349-356.
[15]	刘福广, 陈胜军, 潘红根, 董鹏, 马英民, 黄杰, 杨二娟, 米紫昊, 王艳松, 雒晓涛. 热喷涂复合结构MCrAlY/8YSZ热障涂层的抗剥落能力[J]. 材料研究学报, 2021, 35(4): 313-320.

Viewed

Full text

Abstract

Cited

Shared

Discussed