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| Preparation of Thiol-ene Hydrogels for Extracellular Matrix Simulation |
SU Chenwen1, ZHANG Tingyue1, GUO Liwei1, LI Le1, YANG Ping2, LIU Yanqiu1( ) |
1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
2.School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China |
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Cite this article:
SU Chenwen, ZHANG Tingyue, GUO Liwei, LI Le, YANG Ping, LIU Yanqiu. Preparation of Thiol-ene Hydrogels for Extracellular Matrix Simulation. Chinese Journal of Materials Research, 2021, 35(12): 903-910.
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Abstract The tunable stiffness step-growth hydrogels were prepared by thiol-ene click chemistry between 4 arm-polyethylene glycol-norbornene (4-PEG-NB) and dithiothreitol and supplemented with REDV biopeptide modification for 2D and 3D extracellular matrix (ECM) simulations, in which the 4-PEG-NB macromonomer was produced by the reaction of 4-PEG-OH with norbornene. The results show that the prepared thiol-ene hydrogels present a porous structure, and the thiol-ene cross-linking reaction with high cross-linking efficiency was also confirmed. The tunable Young's modulus of hydrogels could be precisely regulated to 0.79, 2.40, and 4.52 kPa by changing the thiol-ene ratio. As the crosslink ratio of the hydrogels increased, the porosity gradually increased and the swelling rate gradually decreased. The drug release of the hydrogels was faster in the early stage and then gradually slowed down. The cell culture results of 2D and 3D ECM simulations show that the hydrogel had excellent biocompatibility.
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Received: 23 February 2021
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| Fund: National Natural Science Foundation of China(32071320);the Fundamental Research Funds for the Central Universities(201810613085) |
About author: LIU Yanqiu, Tel: 13980462692, E-mail: yqliu@home.swjtu.edu.cn
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