Preparation and Properties of Copper-carrying Polydopamine Coating on Ureteral Stent

doi:10.11901/1005.3093.2021.323

Chinese Journal of Materials Research

2022, Vol. 36

Issue (10): 721-729 DOI: 10.11901/1005.3093.2021.323

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Preparation and Properties of Copper-carrying Polydopamine Coating on Ureteral Stent

LI Jianzhong¹^,², ZHU Boxuan¹, WANG Zhenyu², ZHAO Jing¹(

), FAN Lianhui², YANG Ke¹

^1.Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China
^2.General Hospital of Northern Theater Command, PLA, Shenyang 110083, China

Cite this article:

LI Jianzhong, ZHU Boxuan, WANG Zhenyu, ZHAO Jing, FAN Lianhui, YANG Ke. Preparation and Properties of Copper-carrying Polydopamine Coating on Ureteral Stent. Chinese Journal of Materials Research, 2022, 36(10): 721-729.

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Abstract

Bacteria adhesion and encrustation formation on the surface of ureteral stents have been common complications clinically. Herewith, the Cu grafting polydopamine coating was prepared on 316L stainless steel via polydopamine graft agent assisted electroless Cu plating method, in order to create a coating with performance of anti-infection, anti-stone formation and good biocompatibility for the ureteral stent surface. While the surface morphology, composition and copper ions release of the coatings were assessed by means of scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy and inductively coupled plasma mass spectrometry. Samples were incubated with bacteria and cells respectively, so that to examine the antibacterial ability, encrustation resistance and biocompatibility of coatings. The results show that the coating is evenly distributed. The copper in the coating consists of Cu, CuO and Cu₂O and its thickness is 27.0 nm. The content of releasing copper for each day remains nearly constant after immersion in artificial urine. The antibacterial rates against Escherichia coli and Staphylococcus aureus are 96.2% and 95.9% after incubating for 24 hours, respectively. The contents of calcium and magnesium of samples, which are deposited in the human urine coupled with Staphylococcus aureus for 30 days, are 48.7 mg/L and 235.3 mg/L, respectively, which is significantly lower than that of the control group. Cells proliferation assay shows no cytotoxicity.

Key words: surface and interface in the materials functional coating chemical grafting copper ureteral stent anti-encrustation biocompatibility

Received: 24 May 2021

ZTFLH:

O647.9

Fund: National Key Research and Development Project(2018YFC1105504);Natural Science Foundation of Liaoning Province(2019-BS-255);Science and Technology Program of Liaoning Province(2020JH2/10300159)

About author: ZHAO Jing, Tel: (024)83978251, E-mail: zhaojing@imr.ac.cn

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	Jianzhong LI
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	Ke YANG

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https://www.cjmr.org/EN/10.11901/1005.3093.2021.323 OR https://www.cjmr.org/EN/Y2022/V36/I10/721

Table 1 Chemical component of the artificial urine^[23]

Fig.1 SEM images of samples (a) 316L stainless steel, (b) copper loaded polydopamine coating

Fig.2 Surface scanning images of copper loaded polydopamine coating (a) Fe element, (b) Cu element, (c) O element and (d) C element

Fig.3 AFM morphology of samples (a) 316L stainless steel, (b) polydopamine coating, (c) copper loaded polydopamine coating

Fig.4 Thickness of coating measured by spectroscopic ellipsometer

Fig.5 XPS analysis of copper loaded polydopamine coating (a) full spectrum, (b) N element, (c) C element and (d) Cu element

Table 2 Content of Cu, CuO and Cu₂O of copper loaded polydopamine coating in different sputtering time(mass fraction, %)

Fig.6 Release amounts of Cu ions of different samples in artificial urine for every day

Fig.7 Live bacteria number and antibacterial rate of different samples after incubation for 24 h (a) S.aureus, 316L SS; (b) S.aureus, copper loaded polydopamine coating; (c) E.coli, 316L SS; (d) E.coli, copper loaded polydopamine coating; (e) antibacterial rate

Fig.8 Live bacteria number and antibacterial rate of different samples after immersion for 30 d (a) S.aureus, 316L SS; (b) S.aureus, copper loaded polydopamine coating; (c) E.coli, 316L SS; (d) E.coli, copper loaded polydopamine coating; (e) antibacterial rate

Fig.9 Contents of calcium (a) and magnesium (b) ions on the surface of different materials after immersion in urine for 30 d

Fig.10 Relative proliferation rate of UECs cultured with different material extracts for different times

Fig.11 Dopamine polymerization formula^[26]

1	Garg A, Mishra K G, Bharti P K, et al. Observation of double J stent bacterial colonisation and its correlation with bacteriuria frequency [J]. J. Evol. Med. Dent. Sci., 2017, 6: 5861
2	Szell T, Dressler F F, Goelz H, et al. In vitro effects of a novel coating agent on bacterial biofilm development on ureteral stents [J]. J. Endourol., 2019, 33: 225 doi: 10.1089/end.2018.0616
3	Lombardo R, Tubaro A, Nunzio C D. Ureteral stent encrustation: epidemiology, pathophysiology, management and current technology [J]. J. Urol., 2021, 205: 68 doi: 10.1097/JU.0000000000001343
4	Oderda M, Lacquaniti S, Fasolis G. Allium stent for the treatment of a malignant ureteral stenosis: a paradigmatic case [J]. Urologia J., 2018, 85: 87 doi: 10.5301/uj.5000249
5	Choi J, Chung K J, Choo S H, et al. Long-term outcomes of two types of metal stent for chronic benign ureteral strictures [J]. BMC Urol., 2019, 19: 34 doi: 10.1186/s12894-019-0465-5 pmid: 31060531
6	Rebl H, Renner J, Kram W, et al. Prevention of encrustation on ureteral stents: which surface parameters provide guidance for the development of novel stent materials? [J]. Polymers, 2020, 12: 558 doi: 10.3390/polym12030558
7	Roy R, Tiwari M, Donelli G, et al. Strategies for combating bacterial biofilms: a focus on anti-biofilm agents and their mechanisms of action [J]. Virulence, 2018, 9: 522 doi: 10.1080/21505594.2017.1313372 pmid: 28362216
8	Mosayyebi A, Vijayakumar A, Yue Y Q, et al. Engineering solutions to ureteral stents: material, coating and design [J]. Cent. Eur. J. Urol., 2017, 70: 270
9	Riedl C R, Witkowski M, Plas E, et al. Heparin coating reduces encrustation of ureteral stents: a preliminary report [J]. Int. J. Antimicrob. Agents, 2002, 19: 507 doi: 10.1016/S0924-8579(02)00097-3
10	Chew B H, Davoudi H, Li J M, et al. An in vivo porcine evaluation of the safety, bioavailability, and tissue penetration of a ketorolac drug-eluting ureteral stent designed to improve comfort [J]. J. Endourol., 2010, 24: 1023 doi: 10.1089/end.2009.0523 pmid: 20367085
11	Krambeck A E, Walsh R S, Denstedt J D, et al. A novel drug eluting ureteral stent: a prospective, randomized, multicenter clinical trial to evaluate the safety and effectiveness of a ketorolac loaded ureteral stent [J]. J. Urol., 2010, 183: 1037 doi: 10.1016/j.juro.2009.11.035 pmid: 20092835
12	Felício M R, Silveira G G O S, Oshiro K G N, et al. Polyalanine peptide variations may have different mechanisms of action against multidrug-resistant bacterial pathogens [J]. J. Antimicrob. Chemother., 2021, 76: 1174 doi: 10.1093/jac/dkaa560 pmid: 33501992
13	Mishra B, Basu A, Chua R R Y, et al. Site specific immobilization of a potent antimicrobial peptide onto silicone catheters: evaluation against urinary tract infection pathogens [J]. J. Mater. Chem., 2014, 2B: 1706
14	Gultekinoglu M, Sarisozen Y T, Erdogdu C, et al. Designing of dynamic polyethyleneimine (PEI) brushes on polyurethane (PU) ureteral stents to prevent infections [J]. Acta Biomater., 2015, 21: 44 doi: 10.1016/j.actbio.2015.03.037 pmid: 25848724
15	Leigh B L, Cheng E, Xu L J, et al. Antifouling photograftable zwitterionic coatings on PDMS substrates [J]. Langmuir, 2019, 35: 1100 doi: 10.1021/acs.langmuir.8b00838 pmid: 29983076
16	Wang Y X, Sun Y L, Gu Y H, et al. Articular cartilage-inspired surface functionalization for enhanced lubrication [J]. Adv. Mater. Interfaces, 2019, 6:1900180 doi: 10.1002/admi.201900180
17	Ren L, Xu L, Feng J W, et al. In vitro study of role of trace amount of Cu release from Cu-bearing stainless steel targeting for reduction of in-stent restenosis [J]. J. Mater. Sci. Mater. Med., 2012, 23: 1235 doi: 10.1007/s10856-012-4584-8
18	Ren L, Xu X H, Liu H, et al. Biocompatibility and Cu ions release kinetics of copper-bearing titanium alloys [J]. J. Mater. Sci. Technol., 2021, 95: 237 doi: 10.1016/j.jmst.2021.03.074
19	Jin S J, Qi X, Wang T M, et al. In vitro study of stimulation effect on endothelialization by a copper bearing cobalt alloy [J]. J. Biomed. Mater. Res., 2018, 106A: 561
20	Zhao J, Ren L, Zhang B C, et al. In vitro study on infectious ureteral encrustation resistance of Cu-bearing stainless steel [J]. J. Mater. Sci. Technol., 2017, 33: 1604 doi: 10.1016/j.jmst.2017.03.025
21	Zhao J, Cao Z Q, Lin H, et al. In vivo research on Cu-bearing ureteral stent [J]. J. Mater. Sci. Mater. Med., 2019, 30: 83 doi: 10.1007/s10856-019-6285-z
22	Jin Y Y, Zhu Z Q, Liang L, et al. A facile heparin/carboxymethyl chitosan coating mediated by polydopamine on implants for hemocompatibility and antibacterial properties [J]. Appl. Surf. Sci., 2020, 528: 146539 doi: 10.1016/j.apsusc.2020.146539
23	Haddad L. Synthetic urine and method of making same[P]. US Pat, 7109035, 2006
24	Shen F Y. Construction of copper mediated in situ nitric oxide-generating coating for cardiovascular interventional devices [D]. Chengdu: Southwest Jiaotong University, 2016
	申芳瑜. 用于血管介入器械表面改性的铜介导原位催化产生一氧化氮涂层的构建 [D]. 成都: 西南交通大学, 2016
25	Xu C H, Tian M, Liu L, et al. Fabrication and properties of silverized glass fiber by dopamine functionalization and electroless plating [J]. J. Electrochem. Soc., 2012, 159: D217 doi: 10.1149/2.056204jes
26	Wang L R, Guan H Y, Chen S S, et al. Preparation and antibacterial function of an Cu-bearing chitosan coating on silicone rubber surface [J]. Chin. J. Mater. Res., 2020, 34: 575 doi: 10.11901/1005.3093.2020.047
	王立蓉, 关宏宇, 陈姗姗等. 硅橡胶表面壳聚糖载铜凝胶涂层的制备及其抗菌功能 [J]. 材料研究学报, 2020, 34: 575 doi: 10.11901/1005.3093.2020.047
27	Shao H. Construction of multilyered composites from poly (dopamine) modified carbon nanotubes and the interactions with cells [D]. Guangzhou: Jinan University, 2017
	邵晗. 聚多巴胺修饰碳纳米管静电叠层复合膜的制备及细胞作用研究 [D]. 广州: 暨南大学, 2017
28	Nandakumar R, Santo C E, Madayiputhiya N, et al. Quantitative proteomic profiling of the Escherichia coli response to metallic copper surfaces [J]. Biometals, 2011, 24: 429 doi: 10.1007/s10534-011-9434-5 pmid: 21384090
29	Zanzen U, Bovenkamp-Langlois L, Klysubun W, et al. The interaction of copper ions with Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli: an X-ray absorption near-edge structure (XANES) spectroscopy study [J]. Arch. Microbiol., 2018, 200: 401 doi: 10.1007/s00203-017-1454-2
30	Iribarnegaray V, Navarro N, Robino L, et al. Magnesium-doped zinc oxide nanoparticles alter biofilm formation of Proteus mirabilis [J]. Nanomedicine, 2019, 14: 1551 doi: 10.2217/nnm-2018-0420
31	Multanen M, Talja M, Hallanvuo S, et al. Bacterial adherence to silver nitrate coated poly-L-lactic acid urological stents in vitro [J]. Urol. Res., 2000, 28: 327 pmid: 11127712
32	Milo S, Hathaway H, Nzakizwanayo J, et al. Prevention of encrustation and blockage of urinary catheters by Proteus mirabilis via pH-triggered release of bacteriophage [J]. J. Mater. Chem., 2017, 5B: 5403
33	Donlan R M, Costerton J W. Biofilms: survival mechanisms of clinically relevant microorganisms [J]. Clin. Microbiol. Rev., 2002, 15: 167 doi: 10.1128/CMR.15.2.167-193.2002 pmid: 11932229
34	Stickler D J, Jones G L, Russell A D, et al. Control of encrustation and blockage of Foley catheters [J]. Lancet, 2003, 361: 1435 pmid: 12727400

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