Antibacterial Property and in vitro Biocompatibility of a Ti-Zr-Cu Alloy

doi:10.11901/1005.3093.2020.582

Chinese Journal of Materials Research

2021, Vol. 35

Issue (11): 873-880 DOI: 10.11901/1005.3093.2020.582

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Antibacterial Property and in vitro Biocompatibility of a Ti-Zr-Cu Alloy

YU Jiaying¹^,²^,⁴, YANG Xixiang¹^,²^,⁴, ZHAN Desong¹^,²^,⁴(

), YANG Ke³, REN Ling³, WANG Jingren¹^,²^,⁴, XU Jiawei¹^,²^,⁴

^1.Department of Dental Material, School of Stomatology, China Medical University, Shenyang 110002, China
^2.Liaoning Institute of Dental Research, Shenyang 110002, China
^3.Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China
^4.Liaoning Province Oral Diseases Key Laboratory, Shenyang 110002, China

Cite this article:

YU Jiaying, YANG Xixiang, ZHAN Desong, YANG Ke, REN Ling, WANG Jingren, XU Jiawei. Antibacterial Property and in vitro Biocompatibility of a Ti-Zr-Cu Alloy. Chinese Journal of Materials Research, 2021, 35(11): 873-880.

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Abstract

The antibacterial property and in vitro biocompatibility of a novel Ti-Zr-Cu alloy were investigated. Meanwhile, the number of bacterial colonies on the surface of Ti-Zr-Cu alloy was determined by plate co-culture method, and the morphology of nuclear and cytoskeleton, cell apoptosis and adhesion ability on Ti-Zr-Cu alloy were assessed by means of CCK8 cell proliferation detection and phyllopeptide cell staining observation. The results show that the density of bacterial colonies on the blank sample and Ti-Zr alloy surface was very high, while the number of bacterial colonies on the Ti-Zr-Cu alloy surface was very low. The antibacterial rate of Ti-Zr-Cu alloy against Staphylococcus aureus and Escherichia coli reached 98.28% and 97.67%, respectively, showing excellent antibacterial properties. The relative productivity of MC3T3-E1 cultured on Ti-Zr-Cu alloy surface for 1, 4 and 7 days were 168.8%, 109.8% and 106.5%, respectively, which were all higher than 100%, indicating that this alloy had no cytotoxicity. The adhesion and spread of cells on the surface of Ti-Zr-Cu alloy are good, which is conducive to the adhesion and further growth of adherent cells on the surface of Ti-Zr-Cu alloy, indicating that Ti-Zr-Cu alloy has good biocompatibility. Ti-Zr-Cu alloy had no adverse effect on cell apoptosis. The SEM images of the cells on the surface of Ti-Zr-Cu alloy showed that the adhesion of the cells was normal, which also indicated that the cells had good biocompatibility.

Key words: metal material Ti-Zr-Cu alloy antibacterial property biocompatibility

Received: 05 January 2021

ZTFLH:

R318

Fund: National Natural Science Foundation of China(51631009);Science and Technology Plan of Liaoning Province(ZF2019032);Science and Technology Innovation R & D Plan of Shenyang City(19-112-4-028)

About author: ZHAN Desong, Tel: (024)31927713, E-mail: zhandesong@126.com

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	Jiaying YU
	Xixiang YANG
	Desong ZHAN
	Ke YANG
	Ling REN
	Jingren WANG
	Jiawei XU

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https://www.cjmr.org/EN/10.11901/1005.3093.2020.582 OR https://www.cjmr.org/EN/Y2021/V35/I11/873

Table 1 Compositions of Ti-Zr alloy and Ti-Zr-Cu alloy (%, mass fraction)

Table 2 Grade standard and evaluation result of cytoxicity

Fig.1 Photos of colonies of S. aureus and E. coli after co-culture with Ti-Zr alloy and Ti-Zr-Cu alloy for 24 h (a) blank group (S. aureus); (b) Ti-Zr (S. aureus); (c) Ti-Zr-Cu (S. aureus); (d) blank group (E. coli); (e) Ti-Zr (E. coli); (f) Ti-Zr-Cu (E. coli)

Table 3 Antibacterial rates of different materials against S. aureus and E. coli (n=4)

Fig.2 OD values of MC3T3-E1 cells measured by CCK-8 cell proliferation assay (absorbance at 450 nm)

Fig.3 Rhodamine phalloidin backbone staining of MC3T3-E1 cells on surfaces of Ti-Zr (a) and Ti-Zr-Cu (b) alloys after co-culture for 24 h

Fig.4 Flow scatters and bar graph statistical analyses of MC3T3-E1 cells on Ti-Zr and Ti-Zr-Cu alloys after co-culture for 72 h (a) Ti-Zr alloy; (b) Ti-Zr-Cu alloy; (c) normal cell viability; (d) apotoic cell viability

Fig.5 Images of scanning electron microscopy of MC3T3-E1 cells on surfaces of Ti-Zr (a) and Ti-Zr-Cu (b) alloys after co-culture for 72 h (200× and 1000×)

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