In Vitro Biocompatibility and Antibacterial Property of a Novel Magnesium Phosphate Whisker

doi:10.11901/1005.3093.2015.643

Chinese Journal of Materials Research

2016, Vol. 30

Issue (3): 220-228 DOI: 10.11901/1005.3093.2015.643

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In Vitro Biocompatibility and Antibacterial Property of a Novel Magnesium Phosphate Whisker

ZHAO Bing^1,^2,^4,⁵, XU Dake³, SUN Ziqing³, REN Yibin³, ZHAN Desong^1,^2,^4,^5,^**(

), XIAO Keshen^3,^**, YANG Ke³

1. Department of Dental Material, School of Stomatology, China Medical University, Shenyang 110002, China
2. Liaoning Institute of Dental Research, Shenyang 110002, China
3. Institute of Metal Research, Chinese Academic of Sciences, Shenyang 110016, China
4. Liaoning Province Oral Diseases Key Laboratory, Shenyang 110002, China
5. Liaoning Province Oral Diseases Translation Medcicne Research Center, Shenyang 110002, China;

Cite this article:

ZHAO Bing, XU Dake, SUN Ziqing, REN Yibin, ZHAN Desong, XIAO Keshen, YANG Ke. In Vitro Biocompatibility and Antibacterial Property of a Novel Magnesium Phosphate Whisker. Chinese Journal of Materials Research, 2016, 30(3): 220-228.

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Abstract

The in vitro biocompatibility of a novel magnesium phosphate whisker (M-PW) was evaluated by real-time cellular analysis (RTCA) and Annexin-V/PI double marking methods, and its antibacterial property was evaluated by co-culture method. Results show that the in vitro biocompatibility of the M-PW decreased with the increase of M-PW in the suspensions. It possessed excellent in vitro biocompatibility for the suspensions containing 500 μg/mL or lower amount of the M-PW. It had no toxic effect on osteoblast cells for the suspension with 50 and 200 μg/mL of M-PW respectively. The antibacterial efficacy of the suspensions increased with the increasing amount of M-PW. The antibacterial efficacy against Escherichia coli and Staphylococcus aureus achieved 96.84% and 99.93% respectively for the suspension with 500 μg/mL of M-PW, demonstrating that the novel phosphorous-magnesium whisker possesses excellent antibacterial property.

Key words: inorganic non-metallic materials magnesium phosphate whisker biocompatibility antibacterial property apoptosis RTCA

Received: 17 November 2015

ZTFLH:

TB321

Fund: *Supported by Shenyang Science and Technology Project No. F16-206-9-14.

About author: **To whom correspondence should be addressed, Tel: (024)22891863, E-mail: zhandesong@126.com.

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	Bing ZHAO
	Dake XU
	Ziqing SUN
	Yibin REN
	Desong ZHAN
	Keshen XIAO
	Ke YANG

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https://www.cjmr.org/EN/10.11901/1005.3093.2015.643 OR https://www.cjmr.org/EN/Y2016/V30/I3/220

Fig.1 SEM micrograph of phosphorus-magnesium whisker

Table 1 pH of the whisker treated groups and blank group in α-MEM medium without addition of MC3T3-E1 cell (n=5)

Fig.2 Real time cell analysis (RTCA) profiling of the four treated groups of phosphorus-magnesium whisker and the blank group for MC3T3-E1 mouse cells Blank group; 50 μg/mL phosphorus-magnesium whisker; 200 μg/mL phosphorus-magnesium whisker; 500 μg/mL phosphorus-magnesium whisker; 1000 μg/mL phosphorus-magnesium whisker

Table 2 Cell relative growth rate (RGR) of MC3T3-E1 cells treated with 4 different concentrations of phosphorus-magnesium whisker

Table 3 Cytotoxicity levels of MC3T3-E1 cells treated with 4 different concentrations of phosphorus-magnesium whisker

Fig.3 MC3T3-E1 cell apoptosis detected by Annexin V/PI with flow cytometry after 48 h (a) Blank group; (b) 50 μg/mL phosphorus-magnesium whisker; (c) 200 μg/mL phosphorus-magnesium whisker; (d) 500 μg/mL phosphorus-magnesium whisker; (e) 1000 μg/mL phosphorus-magnesium whisker

Table 4 Apoptosis results measured by flow cytometry after 48 h

Table 5 Antibacterial rate against E.coli and S.aureus treated with four different concentrations of phosphorus-magnesium whisker (n=5)

Fig.4 Antibacterial effect of phosphorus-magnesium whisker against E.coli and S.aureus (a) Blank group (E.coli); (b) treated with 50 μg/mL whisker (E.coli); (c) treated with 1000 μg/mL whisker (E.coli); (d) Blank group (S.aureus); (e) treated with 50 μg/mL whisker (S.aureus); (f) treated with 1000 μg/mL whisker (S.aureus)

Fig.5 A schematic illustration of the bactericidal process of phosphorus-magnesium whisker

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