Preparation and Emulsification Properties of Self-assembled Colloidal Particles Based on Alginic Acid

doi:10.11901/1005.3093.2020.455

Chinese Journal of Materials Research

2021, Vol. 35

Issue (10): 761-768 DOI: 10.11901/1005.3093.2020.455

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Preparation and Emulsification Properties of Self-assembled Colloidal Particles Based on Alginic Acid

ZHANG Cuige(

), HU Liang, LU Zuxin, ZHOU Jiahui

School of Chemical and Environmental Engineering, Anhui Polytechnic University, Wuhu 241000, China

Cite this article:

ZHANG Cuige, HU Liang, LU Zuxin, ZHOU Jiahui. Preparation and Emulsification Properties of Self-assembled Colloidal Particles Based on Alginic Acid. Chinese Journal of Materials Research, 2021, 35(10): 761-768.

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Abstract

Self-assembled colloidal particles were prepared via electrostatic interaction between alginic acid (ALG) and lysozyme (Lys), and the effect of the mass ratio of lysozyme to alginic acid on the properties of colloidal particles was investigated in order to obtain colloidal particles at the optimal mass ratio. The size and morphology of colloidal particles were characterized by nanometer particle size analyzer and transmission electron microscope. The results show that the colloidal particles are spherical with particle size of about 143 nm. The colloidal particles can be assembled at the oil-water interface to stabilize the oil-in-water Pickering emulsion. The effects of pH value and salt concentration on the properties and the emulsifying properties of colloidal particles were investigated in detail. The results show that as the pH value increases, the colloidal particles and emulsion droplet size both gradually increase, and the emulsification performance gradually decreases; as the salt concentration increases the colloidal particles and emulsion droplet size first decreases and then increases, while the emulsification performance increases first and then decreases. The effect of pH and salt concentration on the activity of lysozyme in the emulsion was further investigated. It follows that the prepared emulsions are all active to a certain extent .

Key words: organic polymer materials pickering emulsion self-assembly enzyme activity

Received: 29 October 2020

ZTFLH:

O636.1

Fund: National Natural Science Foundation of China(51703001);Natural Science Foundation of the Higher Education Institutions of Anhui Province(KJ2017A115);Innovation and Entrepreneurship Training Plan of College Students in Anhui Province(S202010363259)

About author: ZHANG Cuige, Tel: (0553)2871254, E-mail: zcg17131@ahpu.edu.cn

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	Cuige ZHANG
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	Jiahui ZHOU

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https://www.cjmr.org/EN/10.11901/1005.3093.2020.455 OR https://www.cjmr.org/EN/Y2021/V35/I10/761

Fig.1 Preparation of Lys/ALG colloidal particles and schematic diagram of secondary assembly at the oil-water interface

Fig.2 Variation of particle size, ζ-potential and polydispersity of Lys/ALG colloidal particles with mass ratio WR

Fig.3 Particle size distribution, Apparent photo (left inset) and TEM image (right inset) of Lys/ALG colloidal particles, mass ratio WR 3.75∶1, the concentration of colloidal particles is 0.625 mg/mL

Fig.4 Variation of colloidal particle size and ζ-potential with pH, WR 3.75∶1, the concentration of colloidal particles is 0.625 mg/mL

Fig.5 Digital photographs of emulsions and microscope photographs of emulsion droplets with different pH (a); average particle diameter and polydispersity of emulsion droplets as a function of pH (b); average particle diameter of emulsion droplets as a function of time (c). The emulsions were incubated 1 day after homogenization. WR 3.75∶1, the concentration of colloidal particles is 0.625 mg/mL

Fig.6 Variation of colloidal particle size and ζ-potential with salt concentration, pH 5.9, WR 3.75∶1, the concentration of colloidal particles is 0.625 mg/mL

Fig.7 Digital photographs of emulsions and microscope photographs of emulsion droplets with different salt concentrations (a); average particle diameter and polydispersity of emulsion droplets as a function of salt concentration (b); average particle diameter of emulsion droplets as a function of time (c). The emulsions were incubated 1 day after homogenization. pH 5.9, WR 3.75∶1, the concentration of colloidal particles is 0.625 mg·mL^-1

Fig.8 Emulsification properties of colloidal particles with different oil-water ratios. (a) digital photo of emulsion and microscope photo of emulsion droplets; (b) average size and polydispersity of emulsion droplets. The emulsion was prepared for 5 hours. pH 5.9, WR 3.75∶1, the concentration of colloidal particles is 0.625 mg/L

Fig.9 Activity of native Lys, Lys/HA colloidal particles and the corresponding emulsions. The emulsions were incubated 1 day after homogenization. The concentration of Lys is 0.625 mg m/L

Fig.10 Activity of Lys in emulsions with different pH (a); activity of Lys in emulsions with different salt concentrations, pH 5.9 (b). The emulsions were incubated 1 day after homogenization. The concentration of Lys is 0.625 mg/mL

1	Rousseau D, Hodge S M. Stabilization of water-in-oil emulsions with continuous phase crystals [J]. Colloids Surf., 2005, 260A: 229
2	Dai L, Sun C X, Wei Y, et al. Characterization of Pickering emulsion gels stabilized by zein/gum arabic complex colloidal nanoparticles [J]. Food Hydrocoll., 2018, 74: 239
3	Riess G. Micellization of block copolymers [J]. Prog. Polym. Sci., 2003, 28: 1107
4	Fujii S, Cai Y, Weaver J V M, et al. Syntheses of shell cross-linked micelles using acidic ABC triblock copolymers and their application as pH-responsive particulate emulsifiers [J]. J. Am. Chem. Soc., 2005, 127: 7304
5	Yi C L, Yang Y Q, Zhu Y, et al. Self-Assembly and emulsification of poly{[styrene-alt-maleic acid]-co- [styrene-alt-(N-3,4-dihydroxyphenylethylmaleamic acid)]} [J]. Langmuir, 2012, 28: 9211
6	Liu X Y, Yi C L, Zhu Y, et al. Pickering emulsions stabilized by self-assembled colloidal particles of copolymers of P(St-alt-MAn)-co-P(VM-alt-MAn) [J]. J. Colloid Interface Sci., 2010, 351: 315
7	Yi C L, Sun J H, Zhao D H, et al. Influence of photo-cross-linking on emulsifying performance of the self-assemblies of poly(7-(4-vinylbenzyloxyl)-4-methylcoumarin-co-acrylic acid [J]. Langmuir, 2014, 30: 6669
8	Liu X Y, Wang Y H, Yi C L, et al. Self-assembly and emulsification behavior of photo-sensitive p(St/CS-alt-MA) copolymer [J]. Acta Chim. Sin., 2009, 67: 447
	刘晓亚, 王益华, 易成林等. 探索光敏性共聚物P(St/CS-alt-MA)的自组装及其乳化行为 [J]. 化学学报, 2009, (67): 447
9	Liu N, Yi C L, Sun J H, et al. Structure and emulsification performance of amphiphilic copolymer self-Assembled micelles [J]. Acta Phys. Chim. Sin., 2013, 29: 327
	刘娜, 易成林, 孙建华等. 双亲性无规共聚物自组装胶束结构及其乳化性能 [J]. 物理化学学报, 2013, (29): 327
10	Tzoumaki M V, Moschakis T, Kiosseoglou V, et al. Oil-in-water emulsions stabilized by chitin nanocrystal particles [J]. Food Hydrocoll., 2011, 25: 1521
11	Song X Y, Pei Y Q, Qiao M W, et al. Preparation and characterizations of Pickering emulsions stabilized by hydrophobic starch particles [J]. Food Hydrocoll., 2015, 45: 256
12	Fujii S, Aichi A, Muraoka M, et al. Ferritin as a bionano-particulate emulsifier [J]. J. Colloid Interface Sci., 2009, 338: 222
13	Hudson D, Margaritis A. Biopolymer nanoparticle production for controlled release of biopharmaceuticals [J]. Crit. Rev. Biotechnol., 2014, 34: 161
14	Cheng Y, Yu S L, Zhen X, et al. Alginic acid nanoparticles prepared through counterion complexation method as a drug delivery system [J]. ACS Appl. Mater. Interfaces, 2012, 4: 5325
15	Drury J L, Mooney D J. Hydrogels for tissue engineering, scaffold design variables and applications [J]. Biomaterials, 2003, 24: 4337
16	Ercan D, Demirci A. Production of human lysozyme in biofilm reactor and optimization of growth parameters of Kluyveromyces lactis K7 [J]. Appl. Microbiol. Biotechnol., 2013, 97: 6211
17	Takahashi D, Uchida K, Izumi T. Activities of lysozyme complexed with polysaccharides and potassium poly (vinyl alcohol sulfate) with various degrees of esterification [J]. Polym. Bull., 2011, 67: 741
18	Fuenzalida J P, Nareddy P K, Moreno-Villoslada I, et al. On the role of alginate structure in complexing with lysozyme and application for enzyme delivery [J]. Food Hydrocoll., 2016, 53: 239
19	Parry R M, Chandan R C, Shahani K M. A rapid and sensitive assay of muramidase [J]. Exp. Biol. Med., 1965, 119: 384
20	Schatz C, Lucas J M, Viton C, et al. Formation and properties of positively charged colloids based on polyelectrolyte complexes of biopolymers [J]. Langmuir, 2004, 20: 7766
21	Zhang C G, Zhu Y, Luo J, et al. Self-assembled papain/HA-Phe composite nanoparticles and emulsification properties [J]. Acta Polymer. Sin., 2016, (7): 963
	张翠歌, 朱叶, 罗静等. Papain/HA-Phe自组装复合纳米粒子及乳化性能 [J]. 高分子学报, 2016, (7): 963
22	Liu H, Wang C Y, Zou S W, et al. Simple, reversible emulsion system switched by pH on the basis of chitosan without any hydrophobic modification [J]. Langmuir, 2012, 28: 11017
23	Manocha B, Margaritis A. Controlled release of doxorubicin from doxorubicin/γ-polyglutamic acid ionic complex [J]. J. Nanomater., 2010, 2010: 780171
24	Mclaren A D, Estermann E F. Influence of pH on the activity of chymotrypsin at a solid-liquid interface [J]. Arch. Biochem. Biophys., 1957, 68: 157
25	Kravchenko N A, Kagramanova K A, Kuznetsov I D. Effect of salts and other factors on lysozyme activity [J]. Biokhimiia, 1967, 32: 618

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