Surface Modification of Hydroxyapatite-Grafted-Chitosan and Biocompatibility Evaluation of CS/HA-G-CS Composite Hydrogel

doi:10.11901/1005.3093.2015.154

Chinese Journal of Materials Research

2015, Vol. 29

Issue (11): 801-806 DOI: 10.11901/1005.3093.2015.154

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Surface Modification of Hydroxyapatite-Grafted-Chitosan and Biocompatibility Evaluation of CS/HA-G-CS Composite Hydrogel

Shenyu YANG¹,Sanyuan TANG²,Manseng TAM³,Rong ZENG¹,Hui YANG²,Hsiungpei HUANG³,Katsang HA⁴,Mei TU^1,^**(

)

1. Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China
2. Zhuhai People’s Hospital, Guangzhou, Zhuhai 519000, China
3. Centro Medico Ian Wo, Macau, China
4. Centro Hospitalar Conde de São Januário, Macau, China

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Shenyu YANG,Sanyuan TANG,Manseng TAM,Rong ZENG,Hui YANG,Hsiungpei HUANG,Katsang HA,Mei TU. Surface Modification of Hydroxyapatite-Grafted-Chitosan and Biocompatibility Evaluation of CS/HA-G-CS Composite Hydrogel. Chinese Journal of Materials Research, 2015, 29(11): 801-806.

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Abstract

For further improvement of the compatibility between chitosan(CS) and hydroxyapatite (HA), surface modification of HA was carried out with CS to prepare hydroxyapatite-grafted-chitosan (HA-g-CS) The results of FT-IR, TGA and XRD show that CS was successfully grafted onto HA surface, SEM observation showed that the dispersity of HA-g-CS in the CS matrix was significantly improved, and the CS/HA-g-CS hydrogel exhibited much more better compression performance than that of the CS/HA. The biocompatibility evaluation showed that the CS/HA-g-CS composite hydrogel showed no cytotoxicity and implantation safety in vitro according with the requirement of national standards, and was expected to be served as a potential scaffold material applied in tissue engineering.

Key words: organic polymer materials chitosan hydroxyapatite surface modification composite hydrogel biocapatibility

Received: 25 March 2015

Fund: *Supported by the Science and Technology Development Funds of Macau No. 072/2013/A, Industrial Technology Research and Development Funds of Guangdong Province No. 2013B021800115, the Project of Science and technology industry and Information of Zhuhai No. 2013032218, the Key Project of Department of Education of Guangdong Province No. CXZD1015, Science and Technology Program of Guangzhou No. 201508020035.

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	Articles by authors
	Shenyu YANG
	Sanyuan TANG
	Manseng TAM
	Rong ZENG
	Hui YANG
	Hsiungpei HUANG
	Katsang HA
	Mei TU

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https://www.cjmr.org/EN/10.11901/1005.3093.2015.154 OR https://www.cjmr.org/EN/Y2015/V29/I11/801

Fig.1 Schematic of steps used to chemically bond chitosan to the hydroxyapatite

Fig.2 XRD patterns of the HA and HA-g-CS powder

Fig.3 FT-IR spectra of HA(a), HA-APTES(b), HA-APTES-GD(c) and HA-g-CS(d)

Fig.4 Thermogravimetric analysis of HA and HA-g-CS (a) HA, (b) HA-g-CS

Fig.5 SEM image of CS/HA and CS/HA-g-CS xerogel

Tab1e 1 Compression strength of the Gels

Tab1e 2 Result for MTT cytotoxicity of CS/HA-g-CS

Fig.6 Observation of cell morphological (a) CS/HA-g-CS, (b) Negative control, (c) Positive control

Fig.7 HE staining photos at different times: a-1week; b-4week; c-12week

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