The evolution of microstructure and texture at different temperatures and its effect on room temperature strength in Ti60 Ti-alloy plates were investigated in the present work. There was no perceivable change of the microstructure or texture after heat treatment at 700?C compared with those of the as-rolled plate. In α+β and β phase regions equiaxed primary α grains shrank and ultimately transformed to secondary α phase, and T-type texture was replaced by a new type of texture with temperature increasing. It was indicated that whether new texture formed or not was significantly affected by the percentage of primary α phase during α→β→α phase transformation: by high percentage, the primary α phase strongly induced the secondary α phase to be with the similar orientation, thereby nearly no change of the texture; by low or zero percentage, part of the formed secondary α phase with new orientation, which was hardly affected by the primary α phase and was inferred as results of α variants selection dominated by texture of β grains formed during rolling at high temperature. Room temperature strength was mainly affected by the substructure: heat treatment in the α phase region didn't eliminate the substructure, the room temperature strength is comparable to that of the as-rolled plates; heat treatment in/above α+β phase field eliminated the substructure, resulting in large reduction of room temperature strength compared with the as-rolled plates. After eliminating the substructure, the room temperature strength was impacted by the microstructure: similar room temperature strength of plates heat treated in low and high α+β phase region is related to the limited effect of the percentage of equiaxed primary α phase on the strength; the room temperature strength decreased after heat treatment in β phase region, and the decrease amplitude in certain direction was remarkably affected by texture. The degree of room temperature anisotropy was influenced by the texture and substructure: higher strength exhibited along the crystallographic c axis; while the substructure weakened the influence of the texture on anisotropy, resulting in stronger anisotropy in plates heat treated in/above α+β phase region.