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| Preparation of the thiophiolic paramagnetic polymer microsphere and its isolation of the antibody from human serum |
| QIAN Hao; LI Chunjiao; ZHANG Yingxue; LIN Zhiyong |
| College of Material Science and Engineering; Huaqiao University; Quanzhou 362021 |
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Cite this article:
QIAN Hao LI Chunjiao ZHANG Yingxue LIN Zhiyong. Preparation of the thiophiolic paramagnetic polymer microsphere and its isolation of the antibody from human serum. Chin J Mater Res, 2009, 23(1): 93-97.
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Abstract The paramagnetic polymer particles with the size range of 0.6–20 μm were synthesized by microsuspension copolymerization with vinyl acetate(VAc) and divinylbenzene(DVB) in the presence of modified magnetite nanoparticles. Evident paramagnetic behavior was observed and saturation magnetization reached 16.7 emu·g−1 at room temperature. These magnetic microspheres were first activated by divinylsulfone, and then modified with mercaptoethanol. By using these thiophilic magnetic microspheres, immunoglobulin G (IgG) could be directly isolated from human serum. This thiophilic magnetic adsorbent performed an evident salt-dependent adsorption behavior for IgG. Due to their strong specificity towards IgG under high salt concentration, the absorbed antibodies could be extracted with high purity. The main factors to influence the isolation, such as the ion types, the ion concentration and the temperature, were also investigated. The technology has great potential to purify antibodies from human serum.
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Received: 25 June 2008
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| Fund: Supported by National Natural Science Foundation of China Nos.50603007 and 50673030 and the Program for New Century Excellent Talents in University of Fujian Province No.07FJRC02. |
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