Preparation of Immobilized Laccase onto Framework-like Material of Metal-organic Compound and Its Degradation for Catechol

doi:10.11901/1005.3093.2021.244

Chinese Journal of Materials Research

2022, Vol. 36

Issue (10): 793-800 DOI: 10.11901/1005.3093.2021.244

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Preparation of Immobilized Laccase onto Framework-like Material of Metal-organic Compound and Its Degradation for Catechol

HOU Yutong¹, LI Xin¹, LI Dawei², WEI Qufu¹(

)

^1.Key Laboratory of Eco-Textiles, Jiangnan University, Wuxi 214122, China
^2.College of Textile and Clothing, Nantong University, Nantong 226019, China

Cite this article:

HOU Yutong, LI Xin, LI Dawei, WEI Qufu. Preparation of Immobilized Laccase onto Framework-like Material of Metal-organic Compound and Its Degradation for Catechol. Chinese Journal of Materials Research, 2022, 36(10): 793-800.

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Abstract

Laccase (LAC) was immobilized onto a framework-like material of metal-organic compound (ZIF-90) by means of internal encapsulation and surface physical-adsorption, herewith, two composites of LAC@ZIF-90 and LAC-ZIF-90 were prepared respectively. The structure, property, stability and degradation performance for catechol of the free- and immobilized-laccase were comparatively characterized by means of scanning electron microscopy (SEM), laser confocal electron microscopy (CLSM), X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), fluorescence analysis and high performance liquid chromatography (HPLC) etc. The results show that: laccase molecules were fixed on the surface and inside of ZIF-90, and the molecular structure was stable before and after being fixed; the removal rates for catechol by lac-ZIF-90 and LAC@ZIF-90 were 97% and 19.4%, respectively for the solution with proper pH value, whilst by vibrating for 6 h; the lac-ZIF-90 and LAC@ZIF-90 still showed high vitality after repeatedly used for 5 times; it is especially worthy that the removal rate of catechol was higher than 78% even after repeatedly used for 5 times for the lac-ZIF-90.

Key words: composits metal organic framework materials laccase immobilization catechol degradation

Received: 15 April 2021

ZTFLH:

TB332

Fund: Natural Science Foundation of Jiangsu Province(BK20180628);Fundamental Research Funds for the Central Universities(JUSRP51907A);Priority Academic Program Development of Jiangsu Higher Education Institutions([2014]37)

About author: WEI Qufu, Tel: 13771106262, E-mail: qfwei@jiangnan.edu.cn

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	Yutong HOU
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https://www.cjmr.org/EN/10.11901/1005.3093.2021.244 OR https://www.cjmr.org/EN/Y2022/V36/I10/793

Fig.1 Schematic illustration showing the synthesis of LAC@ZIF-90 and LAC-ZIF-90

Fig.2 SEM images of ZIF-90 (a), LAC@ZIF-90 (b) and LAC-ZIF-90 (c)

Fig.3 CLSM showing the fluorescence, bright field and overlay images of LAC-ZIF-90 (a, b, c) and LAC@ZIF-90 (d, e, f)

Fig.4 XRD spectra of ZIF-90、LAC@ZIF-90 and LAC-ZIF-90

Fig.5 FTIR spectra of ZIF-90、LAC@ZIF-90、LAC-ZIF-90 and free LAC

Fig.6 Fluorescence spectroscopy of ZIF-90、LAC@ZIF-90、LAC-ZIF-90 and free LAC

Table 1 Porous characteristic of free LAC,LAC-ZIF-90 and LAC@ZIF-90

Fig.7 Effect of pH on the activity of immobilized laccase

Fig.8 Effect of temperature on the activity of immobilized laccase

Fig.9 Removal of catechol by free laccase、immobilized laccase and ZIF-90

Fig.10 Reusability of immobilized laccase

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