Performance of 3D Tissue Engineering Scaffolds of Nanocellulose/High Cationic Polymers Composite

doi:10.11901/1005.3093.2014.293

Chinese Journal of Materials Research

2015, Vol. 29

Issue (1): 1-9 DOI: 10.11901/1005.3093.2014.293

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Performance of 3D Tissue Engineering Scaffolds of Nanocellulose/High Cationic Polymers Composite

Aimin TANG(

),Yuan LIU,Shan ZHAO

State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China

Cite this article:

Aimin TANG,Yuan LIU,Shan ZHAO. Performance of 3D Tissue Engineering Scaffolds of Nanocellulose/High Cationic Polymers Composite. Chinese Journal of Materials Research, 2015, 29(1): 1-9.

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Abstract

Three-dimensional (3D) tissue engineering scaffolds were prepared by compounding nanocellulose with polyacrylic cationic polymer and polyethylene amine cationic polymer respectively. The structural morphology of the scaffolds was characterized by scanning electron microscopy (SEM).The influence of the relative molecular mass and dosages of the polymers on the pore structure of the scaffolds was investigated, while a new method for fast measuring the porosity of the scaffolds was established based on SEM image processing. The water retention value (WRV) of the scaffolds was also measured. Results show that the porosity of all the nanocellulose 3D tissue engineering scaffolds is larger than 90％. The porosity value obtained by the new image processing method is close to that measured according to Archimedes principle with a difference less than 5%, which indicated that this method was reliable. All the 3D scaffolds have high WRV (>200％). Both the porosity and WRV of the 3D scaffolds can be adjusted by varying the species and dosage of polymers. Therewith the nanocellulose 3D tissue engineering scaffolds may optionally be prepared to meet the requirement for tissue and cell culture.

Key words: composites nanocellulose tissue engineering scaffold image processing

Received: 18 June 2014

Fund: *Supported by National Basic Research Program of China No. 2010CB732206.

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	Aimin TANG
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	Shan ZHAO

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https://www.cjmr.org/EN/10.11901/1005.3093.2014.293 OR https://www.cjmr.org/EN/Y2015/V29/I1/1

Fig.1 AFM image of nanocellulose

Fig.2 SEM images of scaffolds prepared by compounding nanocellulose with different percentage of polymer A. (a) 0; (b, f) 0.79%; (c, g) 1.57%; (d, h) 3.85%; (e, i) 7.41% (a-e, surface section; f-i, cross section)

Fig.3 SEM images of scaffolds prepared by compounding nanocellulose with different percentage of polymer B. (a, e) 0.79%; (b, f) 1.57%; (c, g) 3.85%; (d, h) 7.41% (a-d, surface section; e-h, cross section)

Fig.4 Porosity measured by liquid displacement experiment for nanocellulose/polymer A and nanocellulose/polymer B compound scaffolds

Table 1 Porosity data of the nanocellulose/polymer A compound scaffolds measured by liquid displacement experiment and image processing method

Table 2 Porosity data of the nanocellulose/polymer B compound scaffolds measured by liquid displacement experiment and image processing method

Fig.5 SEM images after threshold segmentation (mass percentage of polymers: (a, e) 0.79%, (b, f) 1.57%, (c, g) 3.85%, (d, h)7.41%; a-d, polymer A; e-h, polymer B)

1	ZHANG Anxiong,LV Delong, ZHONG Wei, CHENG Weizhuang, DU Qiangguo, Study advances in natural biomaterials for tissue engineering, Beijing Biomedical Engineering, 24(5), 387(2005)
1	(张安兄, 吕德龙, 钟伟, 程为庄, 杜强国, 天然生物材料构建组织工程支架的研究进展,?北京生物医学工程, 24(5), 387(2005))
2	R. Langer, J. P. Vacanti,Tissue engineering, Science, 260(5110), 920(1993)
3	J. M. Holzwarth, P. X. Ma,Biomimetic nanofibrous scaffolds for bone tissue engineering,?Biomaterials, 32(36), 9622(2011)
4	Michael Keeney, Janice H. Lai,Fan Yang, Recent progress in cartilage tissue engineering, Current Opinion in Biotechnology, 22(5), 734(2011)
5	D. Y. Lewitus, J. Landers, J. R. Branch, K. L. Smith, G. Callegari, J. Kohn, A. V. Neimark,Biohybrid carbon nanotube/agarose fibers for neural tissue engineering, Advanced Functional Materials, 21(14), 2624(2011)
6	S. He, T. Xia, H. Wang, L. Wei, X. Luo, X. Li,Multiple release of polyplexes of plasmids VEGF and bFGF from electrospun fibrous scaffolds towards regeneration of mature blood vessels, Acta Biomaterialia, 8(7), 2659(2012)
7	S. B?ttcher-Haberzeth, T. Biedermann, A. S.Klar, L. Pontiggia, J. Rac, D. Nadal, M. Meuli,Tissue engineering of skin: human tonsil-derived mesenchymal cells can function as dermal fibroblasts, Pediatric Surgery International, 30(2), 213(2014)
8	B. Andrée, A. B?r, A. Haverich, A. Hilfiker,Small intestinal submucosa segments as matrix for tissue engineering: review, Tissue Engineering Part B: Reviews, 19(4), 279(2013)
9	T. Saito, M. Hirota, N. Tamura, S. Kimura, H. Fukuzumi, L. Heux, A. Isogai,Individualization of nano-sized plant cellulose fibrils by direct surface carboxylation using TEMPO catalyst under neutral conditions, Biomacromolecules, 10(7), 1992(2009)
10	S. Iwamoto, A. N. Nakagaito, H. Yano,Nano-fibrillation of pulp fibers for the processing of transparent nanocomposites, Applied Physics A, 89(2), 461(2007)
11	T. Lu, Q. Li, W. Chen, H. Yu,Composite aerogels based on dialdehyde nanocellulose and collagen for potential applications as wound dressing and tissue engineering scaffold, Composites Science and Technology, 94, 132(2014)
12	I. Siró, D. Plackett,Microfibrillated cellulose and new nanocomposite materials: a review, Cellulose, 17(3), 459(2010)
13	R. J. Moon, A. Martini, J. Nairn, J. Simonsen, J. Youngblood,Cellulose nanomaterials review: structure, properties and nanocomposites, Chemical Society Reviews, 40(7), 3941(2011)
14	E. M. Fernandes, R. A. Pires, J. F. Mano, R. L. Reis,Bionanocomposites from lignocellulosic resources: Properties, applications and future trends for their use in the biomedical field, Progress in Polymer Science, 38(10), 1415(2013)
15	Jiankang Song,Aimin Tang, Tingting Liu, Jufang Wang, Fast and continuous preparation of high polymerization degree cellulose nanofibrils and their three-dimensional macroporous scaffold fabrication, Nanoscale, 5(6), 2482(2013)
16	F J O'Brien,Biomaterials & scaffolds for tissue engineering, Materials Today, 14(3), 88(2011)
17	C. M. Murphy, M. G. Haugh, F. J. O'Brien,The effect of mean pore size on cell attachment, proliferation and migration in collagen–glycosaminoglycan scaffolds for bone tissue engineering, Biomaterials, 31(3), 461(2010)
18	B. J. Story, W. R. Wagner, D. M. Gaisser, S. D. Cook, A. M. Rust-Dawicki,In vivo performance of a modified CSTi dental implant coating, International Journal of Oral & Maxillofacial Implants, 13(6), 749(1998)
19	G. Shi, Q. Cai, C. Wang, N. Lu, S. Wang, J. Bei,Fabrication and biocompatibility of cell scaffolds of poly (L‐lactic acid) and poly (L‐lactic‐co‐glycolic acid), Polymers for Advanced Technologies, 13(3-4), 227(2002)
20	LIU Qi,HU Yafei, XIONG Jianjun, Experimental study on porosity measurement on graphite porous materials, Lubrication Engineering, 35(10), 99(2010)
20	(刘颀, 胡亚非, 熊建军, 石墨多孔材料孔隙率测定方法研究, 润滑与密封, 35(10), 99(2010))
21	S. Maria,Methods for porosity measurement in lime-based mortars, Construction and Building Materials, 24(12), 2572(2010)
22	TANG Chaosheng,SHI Bin, WANG Baojun, Factors affecting analysis of soil microstructure using SEM, Chinese Journal of Geotechnical Engineering, 30(4), 560(2008)
22	(唐朝生, 施斌, 王宝军, 基于SEM土体微观结构研究中的影响因素分析, 岩土工程学报, 30(4), 560(2008))
23	ZHOU Ming,WANG Hongbo, WANG Yinli, GAO Weidong, Characterization of porosity of nanofiber membrane based on image processing technology, Journal of Textile Research, 33(1), 20(2012)
23	(周明, 王鸿博, 王银利, 高卫东, 基于图像处理技术的纳米纤维膜孔隙率表征, 纺织学报, 33(1), 20(2012))
24	D. Depan, P. K. C. Venkata Surya, B. Girase, R. D. K. Misra,Organic/inorganic hybrid network structure nanocomposite scaffolds based on grafted chitosan for tissue engineering, Acta biomaterialia, 7(5), 2163(2011)
25	S. Ucar, P. Yilgor, V. Hasirci, N. Hasirci,Chitosan‐based wet‐spun scaffolds for bioactive agent delivery,?Journal of Applied Polymer Science, 130(5), 3759(2013)
26	S. Attaway,Matlab: A practical introduction to programming and problem solving, Elsevier, (2012)
27	SONG Jiankang,Preparation of cellulose nanofibrils and their application in tissue engineering scaffold, Master degree (South China University of Technology, 2012)
27	(宋建康, 纤维素纳米纤维的制备及其在组织工程支架中的应用, 硕士论文(华南理工大学, 2012))
28	X. Luo, J. Y. Zhu,Effects of drying-induced fiber hornification on enzymatic saccharification of lignocelluloses, Enzyme and Microbial Technology, 48(1), 92(2011)
29	HUANG Jinzhong,LI Xuesheng, LU Zejian, QUAN Daping, In vitro and in vivo study on the degradation and biocompatibility of a poly-DL-lactide(PLE) polymer, modem Rehabilitation, 5(7), 58(2001)
29	(黄金中, 李雪盛, 卢择俭, 全大萍, 新型高孔隙率海绵状聚乳酸支架在软骨组织工程研究和应用中的意义, 现代康复, 5(7), 58(2001))

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