贝壳珍珠质及交叉叠片结构体外类骨磷灰石生长的对比研究

doi:10.11901/1005.3093.2021.690

材料研究学报

2023, Vol. 37

Issue (4): 241-247 DOI: 10.11901/1005.3093.2021.690

研究论文

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贝壳珍珠质及交叉叠片结构体外类骨磷灰石生长的对比研究

季洪梅, 王絮, 李小武(

)

东北大学材料科学与工程学院材料各向异性与织构教育部重点实验室沈阳 110819

Comparative Investigations of in vitro Apatites Depositionin Nacre and Crossed-lamellar Structures in Mollusk Shells

JI Hongmei, WANG Xu, LI Xiaowu(

)

School of Material Science and Engineering, Key Laboratory for Anisotropy and Texture of Materials, Northeastern University, Shenyang 110819, China

引用本文:

季洪梅, 王絮, 李小武. 贝壳珍珠质及交叉叠片结构体外类骨磷灰石生长的对比研究[J]. 材料研究学报, 2023, 37(4): 241-247.
Hongmei JI, Xu WANG, Xiaowu LI. Comparative Investigations of in vitro Apatites Depositionin Nacre and Crossed-lamellar Structures in Mollusk Shells[J]. Chinese Journal of Materials Research, 2023, 37(4): 241-247.

全文: PDF(10084 KB) HTML

摘要:

对具有交叉叠片结构的扇贝和珍珠质结构的褶纹冠蚌贝壳进行表面预处理和体外生物活性实验，对比研究了类骨磷灰石在这两种不同微观结构表面的生长。在磷酸缓冲液PBS中浸泡的初始阶段，磷灰石在交错层状结构表面沉积得很紧密，沉积速度较高；而在SBF模拟人体体液中的体外矿化过程中，在交叉叠片结构表面在较短时间内就生成了数量更多的磷灰石。长期浸泡后，两种结构的生物活性没有明显的不同。与珍珠质结构相比，在短时间内交叉叠片结构更有利于类骨磷灰石的生长。

关键词 ：材料科学其它学科, 贝壳, 体外类骨磷灰石成长, 珍珠质, 交叉叠片结构

Abstract：

One specie of Pectinidae with a crossed-lamellar structure and one of Plicata with a nacreous structure were selected as the target materials in this paper. The growth of bone-like apatite on the surfaces of these two kinds of microstructures by a pre-treatment and an in vitro bioactivity assessment was investigated. The apatites were deposited more compactly and more quickly on the surface of crossed-lamellar structure in the preliminary stage of immersion in PBS, as well as in SBF. However, these two kinds of microstructures exhibit identically an excellent bioactivity after a subsequently long-term in vitro mineralization process in SBF. Therefore, in contrast to nacreous structure the crossed-lamellar structure can promote the deposition of the apatites no matter in a short term or in a long term.

Key words： other disciplines of the materials science biological shell apatites deposition nacre crossed-lamellar structure

收稿日期: 2021-12-14

ZTFLH:

TB332

基金资助:国家自然科学基金(51902043);中央高校基科研业务费(N2102007);中央高校基科研业务费(N2102002)

作者简介: 季洪梅，女，1990年生，博士

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https://www.cjmr.org/CN/10.11901/1005.3093.2021.690 或 https://www.cjmr.org/CN/Y2023/V37/I4/241

图1 扇贝的宏观形貌、腐蚀后横截面的微观结构以及褶纹冠蚌的宏观形貌、横截面自然断口形貌、贝壳平面内的腐蚀形貌、矿物板片腐蚀后的微观形貌

图2 扇贝和褶纹冠蚌贝壳样品浸泡PBS前后的XRD谱

图3 扇贝和褶纹冠蚌贝壳样品浸泡在PBS后的SEM照片

图4 扇贝和褶纹冠蚌贝壳样品在PBS中浸泡后表面Ca/P的变化

图5 扇贝和褶纹冠蚌贝壳样品在PBS中浸泡不同时间后溶液中Ca2+与P5+浓度和pH值的变化

图6 扇贝和褶纹冠蚌贝壳样品在PBS预处理后在SBF溶液中浸泡5、10和15 d后的XRD谱

图7 用PBS预处理后扇贝和褶纹冠蚌贝壳样品在SBF溶液中浸泡5、10和15 d的SEM照片

图8 浸泡在SBF中扇贝和褶纹冠蚌贝壳样品表面的Ca/P比值

1	Bogild O B. The shell structure of the mollusks[J]. K. Dan. Vidensk. Selsk. Skr. Naturvidensk. Math. Afd., 1930, 9(5): 232
2	Kobayashi I, Samata T. Bivalve shell structure and organic matrix[J]. Mater. Sci. Eng. C, 2006, 26(4): 692 doi: 10.1016/j.msec.2005.09.101
3	Li H Z, Jin D L, Li R, et al. Structural and mechanical characterization of thermally treated conch shells[J]. JOM, 2015, 67: 720 doi: 10.1007/s11837-015-1330-y
4	Huang Z W, Li X D. Nanoscale structural and mechanical characterization of heat treated nacre[J]. Mater. Sci. Eng. C, 2009, 29(6): 1803 doi: 10.1016/j.msec.2009.02.007
5	Liang Y, Zhao J, Wang L. The structure and mechanical Properties of mollusk shell[J]. Chinese J. Mater. Res., 2007, 21(5): 555
5	梁艳, 赵杰, 王来. 香螺壳的结构和微观力学性能[J]. 材料研究学报, 2007, 21(5): 555
6	Jackson A P, Vincent J F V, Turner R M. Comparison of nacre with other ceramic composites[J]. J. Mater. Sci., 1990, 25: 3173 doi: 10.1007/BF00587670
7	Ji H M, Yang W, Chen D L, et al. Natural arrangement of fiber-like aragonites and its impact on mechanical behavior of mollusk shells: A review[J]. J. Mech. Behav. Biomed. Mater., 2020, 110: 103940 doi: 10.1016/j.jmbbm.2020.103940
8	Sun J, Bhushan B. Hierarchical structure and mechanical properties of nacre: a review[J]. Rsc Adv., 2012, 2(20): 7617 doi: 10.1039/c2ra20218b
9	Li X W, Ji H M, Yang W, et al. Mechanical properties of crossed-lamellar structures in biological shells: A review[J]. J. Mech. Behav. Biomed. Mater., 2017, 74: 54 doi: S1751-6161(17)30211-4 pmid: 28550764
10	Lopez E, Vidal B, Berland S, et al. Demonstration of the capacity of nacre to induce bone formation by human osteoblasts maintained in vitro[J]. Tissue & Cell, 1992, 24(5): 667
11	Atlan G, Balmain N, Berland S, et al. Reconstruction of human maxillary defects with nacre powder: histological evidence for bone regeneration[J]. Comptes Rendus Acad. Sci. Ser. III, 1997, 320(3): 253
12	Lamghari M, Almeida M J, Berland S, et al. Stimulation of bone marrow cells and bone formation by nacre: in vivo and in vitro studies[J]. Bone, 1999, 25(2): 91S doi: 10.1016/S8756-3282(99)00113-1
13	Liao H H, Mutvei H, Hammarström L, et al. Tissue responses to nacreous implants in rat femur: an in situ hybridization and histochemical study[J]. Biomaterials, 2002, 23(13): 2693 pmid: 12059018
14	Yoshimura M, Sujaridworakun P, Koh F, et al. Hydrothermal conversion of calcite crystals to hydroxyapatite[J]. Mater. Sci. Eng. C, 2004, 24(4): 521 doi: 10.1016/j.msec.2004.01.005
15	Yang C L, Chen J T, Jin D D, et al. The bone induction effect of water soluble matrix from nacre powder on human marrow stroma cells[J]. Chin. J. Clin. Anat., 2007, 25(2): 190
15	杨春露, 陈建庭, 金大地等. 珍珠层水溶性提取物对人骨髓基质细胞成骨性分化的诱导作用[J]. 中国临床解剖学杂志, 2007, 25(2): 190
16	Almagro I, Drzymała P, Berent K, et al. New crystallographic relationships in biogenic aragonite: the crossed-lamellar microstructures of mollusks[J]. Cryst. Growth Des., 2016, 16: 2083 doi: 10.1021/acs.cgd.5b01775
17	Wang X, Ji H M, Li X W. Microstructure-related in vitro bioactivity of a natural ceramic of Saxidomus purpuratus shell[J]. Mater. Des., 2018, 139: 512 doi: 10.1016/j.matdes.2017.11.035
18	Kokubo T, Kushitani H, Sakka S, et al. Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W3[J]. J. Biomed. Mater. Res., 1990, 24(6): 721 doi: 10.1002/(ISSN)1097-4636
19	Koutsopoulos S, Dalas E. The effect of acidic amino acids on hydroxyapatite crystallization[J]. J. Cryst. Growth, 2000, 217: 410 doi: 10.1016/S0022-0248(00)00502-9
20	Ni M, Ratner B D. Nacre surface transformation to hydroxyapatite in a phosphate buffer solution[J]. Biomaterials, 2003, 24(4): 4323 doi: 10.1016/S0142-9612(03)00236-9
21	Crenshaw M A. The soluble matrix from Mercenaria mercenaria shell[J]. Biomineralization, 1972, 6: 6

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