Comparative Investigations of in vitro Apatites Depositionin Nacre and Crossed-lamellar Structures in Mollusk Shells

doi:10.11901/1005.3093.2021.690

Chinese Journal of Materials Research

2023, Vol. 37

Issue (4): 241-247 DOI: 10.11901/1005.3093.2021.690

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Comparative Investigations of in vitro Apatites Depositionin Nacre and Crossed-lamellar Structures in Mollusk Shells

JI Hongmei, WANG Xu, LI Xiaowu(

)

School of Material Science and Engineering, Key Laboratory for Anisotropy and Texture of Materials, Northeastern University, Shenyang 110819, China

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JI Hongmei, WANG Xu, LI Xiaowu. Comparative Investigations of in vitro Apatites Depositionin Nacre and Crossed-lamellar Structures in Mollusk Shells. Chinese Journal of Materials Research, 2023, 37(4): 241-247.

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Abstract

One specie of Pectinidae with a crossed-lamellar structure and one of Plicata with a nacreous structure were selected as the target materials in this paper. The growth of bone-like apatite on the surfaces of these two kinds of microstructures by a pre-treatment and an in vitro bioactivity assessment was investigated. The apatites were deposited more compactly and more quickly on the surface of crossed-lamellar structure in the preliminary stage of immersion in PBS, as well as in SBF. However, these two kinds of microstructures exhibit identically an excellent bioactivity after a subsequently long-term in vitro mineralization process in SBF. Therefore, in contrast to nacreous structure the crossed-lamellar structure can promote the deposition of the apatites no matter in a short term or in a long term.

Key words: other disciplines of the materials science biological shell apatites deposition nacre crossed-lamellar structure

Received: 14 December 2021

ZTFLH:

TB332

Fund: National Natural Science Foundation of China(51902043);Fundamental Research Funds for the Central Universities(N2102007);Fundamental Research Funds for the Central Universities(N2102002)

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https://www.cjmr.org/EN/10.11901/1005.3093.2021.690 OR https://www.cjmr.org/EN/Y2023/V37/I4/241

Fig.1 Overall views and microstructures of the Pectinidae (a~d) and Plicata (e~h) shells

Fig.2 XRD patterns of the Pectinidae (a) and Plicata (b) shell samples before and after soaking in PBS

Fig. 3 SEM images showing the surface morphologies of Pectinidae (a~c) and Plicata (d~f) shell samplessoaked in PBS for 1, 3 and 5 days, respectively

Fig.4 Changes in Ca/P ratio of Pectinideaand Plicata shell samples after soaking in PBS

Fig.5 Changes of Ca²⁺ and P⁵⁺ concentrations and pH value of Pectinidea and Plicata shell samples after soaking in PBS for different time

Fig.6 XRD patterns ofPectinidea (a) and Plicata (b) shell samples firstly treated in PBS for 5 d and then soaked in SBF for different times

Fig.7 SEM images showing the surface morphologies of Pectinidea (a~c) and Plicata (d~f) shell samples soaked in SBF for 5, 10 and 15 d, respectively

Fig.8 Changes in Ca/P ratio of Pectinidae and Plicata shell samples after soaking in SBF

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