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Synthesis and Self-assembly Behavior of Amylose-chenodeoxycholic Acid Conjugates |
XIONG Xiaoqin(), ZHANG Hongquan, FANG Chanyu |
Hubei Key Laboratory of Purification and Application of Plant Anticancer Active Ingredients, School of Chemistry and Life Sciences, Hubei University of Education, Wuhan 430205, China |
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Cite this article:
XIONG Xiaoqin, ZHANG Hongquan, FANG Chanyu. Synthesis and Self-assembly Behavior of Amylose-chenodeoxycholic Acid Conjugates. Chinese Journal of Materials Research, 2020, 34(8): 569-574.
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Abstract Amylose-chenodeoxycholic acid conjugates (AMY-CDCA) were prepared using 1-ethyl-3-(3-dimethylaminopropyl)-carbonized diimine (EDC) /N-hydroxysuccinimide (NHS) cross-linking agents. The AMY-CDCA polymer was characterized by FTIR, 1H NMR and ultraviolet spectroscopy. It was shown that CDCA was successfully coupled to the amylose backbone with a molar substitution of 138.15 per 100 glucose units. Self-Assembled micelles were prepared from the AMY-CDCA polymer through dialysis method. The average particle size of the micelles measured by dynamic light scattering method is 224 nm, and the polydispersity index is 0.110. TEM images demonstrated that the micelles are of spherical shape with a core-shell structure. The critical micelle concentration is 2.8×10-3 mg/mL, which was determined using a probe fluorescence technique in the presence of pyrene. Additionally, Nile Red can be encapsulated and stabilized in the hydrophobic core of the micelles.
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Received: 15 January 2020
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Fund: Natural Science Foundation of Hubei Province(2016CFB310) |
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